Rabbit anti-Human ZNF174 Polyclonal Antibody | anti-ZNF174 antibody

ZNF174, ID (ZNF174, ZSCAN8, Zinc finger protein 174, AW-1, Zinc finger and SCAN domain-containing protein 8) (Azide free) (HRP)

Cat. Num. AAA6365748

• Gene Names: ZNF174; ZSCAN8
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ZNF174; Polyclonal Antibody; ID (ZNF174; ZSCAN8; Zinc finger protein 174; AW-1; Zinc finger and SCAN domain-containing protein 8) (Azide free) (HRP); Zinc finger and SCAN domain-containing protein 8; anti-ZNF174 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ZNF174 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ZNF174 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 193-222 amino acids from the Central region of human ZNF174.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ZNF174 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the ZNF174 antibody detected the ZNF174 protein (arrow).)

Product Categories/Family for anti-ZNF174 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

Lamesch, P., et al. Genomics 89(3):307-315(2007). Ivanov, D., et al. Mol. Cell 17(1):137-143(2005). Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005). Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005). Stone, J.R., et al. J. Biol. Chem. 277(7):5448-5452(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 73808088
• NCBI GeneID: 7727
• NCBI Accession #: NP_001027463.1; NP_003441.1
• NCBI GenBank Nucleotide #: NM_001032292.2
• UniProt Accession #: Q15697; Q53Y68; Q9BQ34
• Molecular Weight: 46,455 Da
• NCBI Official Full Name: zinc finger protein 174 isoform b
• NCBI Official Synonym Full Names: zinc finger protein 174
• NCBI Official Symbol: ZNF174
• NCBI Official Synonym Symbols: ZSCAN8
• NCBI Protein Information: zinc finger protein 174; AW-1; zinc finger and SCAN domain-containing protein 8
• UniProt Protein Name: Zinc finger protein 174
• Protein Family: Zinc finger protein
• UniProt Gene Name: ZNF174
• UniProt Synonym Gene Names: ZSCAN8
• UniProt Entry Name: ZN174_HUMAN

Uniprot Description

ZNF174: a transcriptional repressor of the krueppel C2H2-type zinc-finger protein family. Two splice variant isoforms have been described.

Protein type: Transcription factor; C2H2-type zinc finger protein; DNA-binding

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; cytoplasm; nucleus; actin cytoskeleton

Molecular Function: protein homodimerization activity; metal ion binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent