Rabbit anti-Human ZN180 Polyclonal Antibody | anti-ZN180 antibody

ZN180, NT (ZNF180, Zinc finger protein 180, HHZ168) (Azide free) (HRP)

Cat. Num. AAA6365517

• Gene Names: ZNF180; HHZ168
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ZN180; Polyclonal Antibody; NT (ZNF180; Zinc finger protein 180; HHZ168) (Azide free) (HRP); ZNF180; HHZ168; anti-ZN180 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ZN180 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ZN180 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-136 amino acids from the N-terminal region of human ZN180.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ZN180 Antibody (N-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the ZN180 antibody detected the ZN180 protein (arrow).)

Immunohistochemistry (IHC)

(ZN180 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZN180 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ZN180 Antibody (N-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ZN180 antibody

Zinc finger proteins have been shown to interact with nucleic acids and to have diverse functions. The zinc finger domain is a conserved amino acid sequence motif containing 2 specifically positioned cysteines and 2 histidines that are involved in coordinating zinc. Kruppel-related proteins form 1 family of zinc finger proteins. See MIM 604749 for additional information on zinc finger proteins.

Product Categories/Family for anti-ZN180 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

Terracciano, A., et al. Mol. Psychiatry (2008). In press: Grimwood, J., et al. Nature 428(6982):529-535(2004). Shannon, M., et al. Genome Res. 13 (6A), 1097-1110 (2003): Wang, R., et al. Somat. Cell Mol. Genet. 22(3):245-248(1996).

NCBI and Uniprot Product Information

• NCBI GI #: 190570172
• NCBI GeneID: 7733
• NCBI Accession #: NP_037388.2
• NCBI GenBank Nucleotide #: NM_013256.3
• UniProt Accession #: Q9UJW8; Q9P1U2; B2RCN6; Q58F03
• Molecular Weight: 79,111 Da
• NCBI Official Full Name: zinc finger protein 180
• NCBI Official Synonym Full Names: zinc finger protein 180
• NCBI Official Symbol: ZNF180
• NCBI Official Synonym Symbols: HHZ168
• NCBI Protein Information: zinc finger protein 180
• UniProt Protein Name: Zinc finger protein 180
• UniProt Gene Name: ZNF180
• UniProt Entry Name: ZN180_HUMAN