Western Blot (WB)
(Western blot analysis of ZIP1 in mouse kidney tissue lysate with ZIP1 antibody at (A) 1 and (B) 2 μg/mL.)
Rabbit anti-Human, Mouse ZIP1 Polyclonal Antibody | anti-SLC39A1 antibody
ZIP1 Antibody
Gene Names
SLC39A1; ZIP1; ZIRTL
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
ZIP1 Antibody is affinity chromatography purified via peptide column.
Synonyms
ZIP1; Polyclonal Antibody; ZIP1 Antibody; ZIP1 Antibody: ZIP1; ZIRTL; IRT1; CGI-08; CGI-71; Zinc transporter ZIP1; Solute carrier family 39 member 1; ZIP-1; anti-SLC39A1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
At least two isoforms of ZIP1 are known to exist; this antibody will detect both isoforms. ZIP1 antibody is predicted not to cross-react with other ZIP family proteins.
Purity/Purification
ZIP1 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid; ZIP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1 mg/mL (varies by lot)
Applicable Applications for anti-SLC39A1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)
Application Notes
ZIP1 antibody can be used for detection of ZIP1 by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Optimal dilutions for each application to be determined by the researcher.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Optimal dilutions for each application to be determined by the researcher.
Homology
Predicted species reactivity based on immunogen sequence: Bovine: (88%)
Immunogen
ZIP1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human ZIP1.
The immunogen is located within amino acids 40 - 90 of ZIP1.
The immunogen is located within amino acids 40 - 90 of ZIP1.
Positive Control
1) Cat. No. MBS151592 - Mouse Kidney Tissue Lysate
2) Cat. No. MBS154338 - Human Kidney Tissue Slide
2) Cat. No. MBS154338 - Human Kidney Tissue Slide
Preparation and Storage
ZIP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of ZIP1 in mouse kidney tissue lysate with ZIP1 antibody at (A) 1 and (B) 2 μg/mL.)
Western Blot (WB)
(Western blot analysis of ZIP1 in mouse kidney tissue lysate with ZIP1 antibody at (A) 1 and (B) 2 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of ZIP1 in human kidney tissue with ZIP1 antibody at 2.5 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of ZIP1 in human kidney tissue with ZIP1 antibody at 2.5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of ZIP1 in human kidney tissue with ZIP1 antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of ZIP1 in human kidney tissue with ZIP1 antibody at 20 μg/mL.)
Related Product Information for anti-SLC39A1 antibody
ZIP1 Antibody: ZIP1, also known as ZIRTL (zinc-iron regulated transporter-like), is the first mammalian member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. ZIP1 expression is markedly downregulated in a number of cancerous tissues and is thought to function as a tumor suppressor gene in prostate cancer. More recent studies have shown that overexpression of ZIP1 and concomitant increased levels of intracellular zinc in PC-3 cells cause a significant inhibition of NF-κB, leading to down-regulation of the antiapoptotic protein Bcl-2, Bcl-XL, and the apoptosis inhibitor XIAP, reducing the malignant potential of prostate cancer cells in vitro and in vivo.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 13, 36 kDa
Observed: 38 kDa
Observed: 38 kDa
NCBI Official Full Name
zinc transporter ZIP1 isoform a
NCBI Official Synonym Full Names
solute carrier family 39 (zinc transporter), member 1
NCBI Official Symbol
SLC39A1
NCBI Official Synonym Symbols
ZIP1; ZIRTL
NCBI Protein Information
zinc transporter ZIP1; zrt- and Irt-like protein 1; solute carrier family 39 (zinc transporter), member 3
UniProt Protein Name
Zinc transporter ZIP1
Protein Family
UniProt Gene Name
SLC39A1
UniProt Synonym Gene Names
IRT1; ZIP1; ZIRTL; ZIP-1; hZIP1
UniProt Entry Name
S39A1_HUMAN
NCBI Description
This gene encodes a member of the zinc-iron permease family. The encoded protein is localized to the cell membrane and acts as a zinc uptake transporter. This gene has been linked to prostate cancer, breast cancer, and Alzheimer's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]
Uniprot Description
SLC39A1: Mediates zinc uptake. May function as a major endogenous zinc uptake transporter in many cells of the body. Responsible for the rapid uptake and accumulation of physiologically effective zinc in prostate cells. Belongs to the ZIP transporter (TC 2.A.5) family.
Protein type: Transporter; Membrane protein, integral; Membrane protein, multi-pass; Transporter, SLC family
Chromosomal Location of Human Ortholog: 1q21
Cellular Component: endoplasmic reticulum membrane; membrane; integral to membrane; plasma membrane
Molecular Function: inorganic cation transmembrane transporter activity; zinc ion transmembrane transporter activity; receptor binding
Biological Process: transmembrane transport; cation transport
Research Articles on SLC39A1
Similar Products
Product Notes
The SLC39A1 slc39a1 (Catalog #AAA150157) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZIP1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ZIP1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF). ZIP1 antibody can be used for detection of ZIP1 by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application to be determined by the researcher. Researchers should empirically determine the suitability of the SLC39A1 slc39a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZIP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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