Rabbit anti-Human ZIC4 Polyclonal Antibody | anti-ZIC4 antibody

ZIC4, CT (Zinc Finger Protein ZIC 4, Zinc Finger Protein Of The Cerebellum 4) (APC)

Cat. Num. AAA6506456

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: ZIC4; Polyclonal Antibody; CT (Zinc Finger Protein ZIC 4; Zinc Finger Protein Of The Cerebellum 4) (APC); anti-ZIC4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ZIC4.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-ZIC4 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-term region of human ZIC4 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ZIC4 (arrow) using rabbit polyclonal ZIC4 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ZIC4 gene (Lane 2) (Origene Technologies).)

Product Categories/Family for anti-ZIC4 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

1. Ishiguro, A., Biochem. Biophys. Res. Commun. 324(1), 302-307 (2004). 2. Grinberg, I., Nat. Genet. 36(10), 1053-1055 (2004). 3. Aruga, J., Gene 172(2), 291-294 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 22547201
• NCBI GeneID: 84107
• NCBI Accession #: NP_115529
• NCBI GenBank Nucleotide #: NM_032153.5
• UniProt Accession #: Q8N9L1
• Molecular Weight: 36kDa
• NCBI Official Full Name: zinc finger protein ZIC 4 isoform 3
• NCBI Official Synonym Full Names: Zic family member 4
• NCBI Official Symbol: ZIC4
• NCBI Protein Information: zinc finger protein ZIC 4
• UniProt Protein Name: Zinc finger protein ZIC 4
• Protein Family: Zinc finger protein
• UniProt Gene Name: ZIC4
• UniProt Entry Name: ZIC4_HUMAN

NCBI Description

This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. Members of this family are important during development, and have been associated with X-linked visceral heterotaxy and holoprosencephaly type 5. This gene is closely linked to the gene encoding zinc finger protein of the cerebellum 1, a related family member on chromosome 3. Heterozygous deletion of these linked genes is involved in Dandy-Walker malformation, which is a congenital cerebellar malformation. Multiple transcript variants have been identified for this gene. [provided by RefSeq, Dec 2009]

Uniprot Description

ZIC4: Binds to DNA. Belongs to the GLI C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 3q24

Cellular Component: nucleus

Molecular Function: DNA binding; metal ion binding