Rabbit anti-Human ZIC3 Polyclonal Antibody | anti-ZIC3 antibody

ZIC3, NT (Zinc fInger Protein ZIC 3, Zinc Finger Protein Of The Cerebellum 3) (AP)

Cat. Num. AAA6506444

• Gene Names: ZIC3; HTX; HTX1; ZNF203; VACTERLX
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: ZIC3; Polyclonal Antibody; NT (Zinc fInger Protein ZIC 3; Zinc Finger Protein Of The Cerebellum 3) (AP); anti-ZIC3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ZIC3.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-ZIC3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human ZIC3 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-ZIC3 Antibody (N-term) in HL60 cell line lysates (35ug/lane).ZIC3(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ZIC3 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-ZIC3 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

1. Zhu, L., Hum. Mutat. 29(1), 99-105 (2008). 2. Zhu, L., Hum. Mol. Genet. 16(14), 1649-1660 (2007). 3. Bedard, J.E., Hum. Mol. Genet. 16(2), 187-198 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 4507973
• NCBI GeneID: 7547
• NCBI Accession #: NP_003404
• NCBI GenBank Nucleotide #: NM_003413
• UniProt Accession #: O60481; Q14DE5; Q5JY75; B2CNW4
• Molecular Weight: 50,045 Da
• NCBI Official Full Name: zinc finger protein ZIC 3
• NCBI Official Synonym Full Names: Zic family member 3
• NCBI Official Symbol: ZIC3
• NCBI Official Synonym Symbols: HTX; HTX1; ZNF203; VACTERLX
• NCBI Protein Information: zinc finger protein ZIC 3; Zic family member 3 (odd-paired homolog, Drosophila); heterotaxy 1; zinc finger protein 203; zinc finger protein of the cerebellum 3
• UniProt Protein Name: Zinc finger protein ZIC 3
• Protein Family: Zinc finger protein
• UniProt Gene Name: ZIC3
• UniProt Synonym Gene Names: ZNF203
• UniProt Entry Name: ZIC3_HUMAN

Uniprot Description

ZIC3: Acts as transcriptional activator. Required in the earliest stages in both axial midline development and left-right (LR) asymmetry specification. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'. Defects in ZIC3 are the cause of visceral heterotaxy X- linked type 1 (HTX1). A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy X- linked type 1 include dextrocardia, corrected transposition of great arteries, ventricular septal defect, patent ductus arteriosus, pulmonic stenosis, situs inversus viscerum, and asplenia and/or polysplenia. Defects in ZIC3 are a cause of VACTERL association X- linked with or without hydrocephalus (VACTERLX). A syndrome characterized by vertebral anomalies, anal atresia, cardiac malformations, tracheoesophageal fistula, renal anomalies (urethral atresia with hydronephrosis), and limb anomalies (hexadactyly, humeral hypoplasia, radial aplasia, and proximally placed thumb). Some patients may have hydrocephalus. Some cases of VACTERL-H are associated with increased chromosome breakage and rearrangement. Belongs to the GLI C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; C2H2-type zinc finger protein; Transcription factor

Chromosomal Location of Human Ortholog: Xq26.2

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; sequence-specific DNA binding; metal ion binding; transcription factor activity

Biological Process: anterior/posterior pattern formation; transcription, DNA-dependent; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; heart looping; determination of left/right symmetry; lung development

Disease: Vacterl Association, X-linked, With Or Without Hydrocephalus; Heterotaxy, Visceral, 1, X-linked