Rabbit anti-Human ZFYVE20 Polyclonal Antibody | anti-ZFYVE20 antibody

ZFYVE20 polyclonal antibody

Cat. Num. AAA3001688

• Gene Names: ZFYVE20; Rabenosyn-5
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: ZFYVE20; Polyclonal Antibody; ZFYVE20 polyclonal antibody; Rabenosyn-5; 110 kDa protein; FYVE finger-containing Rab5 effector protein rabenosyn-5; Zinc finger FYVE domain-containing protein 20; anti-ZFYVE20 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Rabenosyn-5 Polyclonal Antibody detects endogenous levels of Rabenosyn-5 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Applicable Applications for anti-ZFYVE20 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1/500-1/2000; ELISA: 1/10,000
• Immunogen: The antiserum was produced against synthesized peptide derived from human ZFYVE20. AA range: 145-194
• Preparation and Storage: Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of various cells using Rabenosyn-5 Polyclonal Antibody diluted at 1?1000)

Western Blot (WB)

(Western blot analysis of lysates from HUVEC and Jurkat cells, using ZFYVE20 Antibody. The lane on the right is blocked with the synthesized peptide.)

Western Blot (WB)

(Western blot analysis of the lysates from COLO205 cells using ZFYVE20 antibody.)

Related Product Information for anti-ZFYVE20 antibody

Background: rabenosyn, RAB effector(RBSN) Homo sapiens. This gene encodes a protein that belongs to the FYVE zinc finger family of proteins. The encoded protein interacts with Ras-related proteins that regulate membrane trafficking. A missense mutation in this gene is associated with a defect in the early endocytic pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

NCBI and Uniprot Product Information

• NCBI GI #: 70980547
• NCBI GeneID: 64145
• NCBI Accession #: NP_071735.2
• NCBI GenBank Nucleotide #: NM_022340.2
• UniProt Accession #: Q9H1K0; Q3KP30; Q59EY8; Q8NAQ1
• Molecular Weight: 88,870 Da
• NCBI Official Full Name: rabenosyn-5
• NCBI Official Synonym Full Names: zinc finger, FYVE domain containing 20
• NCBI Official Symbol: ZFYVE20
• NCBI Official Synonym Symbols: Rabenosyn-5
• NCBI Protein Information: rabenosyn-5; 110 kDa protein; zinc finger FYVE domain-containing protein 20; FYVE finger-containing Rab5 effector protein rabenosyn-5; FYVE-finger-containing Rab5 effector protein rabenosyn-5
• UniProt Protein Name: Rabenosyn-5
• UniProt Gene Name: ZFYVE20
• UniProt Entry Name: RBNS5_HUMAN

NCBI Description

This gene encodes a protein that belongs to the FYVE zinc finger family of proteins. The encoded protein interacts with Ras-related proteins that regulate membrane trafficking. A missense mutation in this gene is associated with a defect in the early endocytic pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Uniprot Description

ZFYVE20: Rab4/Rab5 effector protein acting in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Required for endosome fusion either homotypically or with clathrin coated vesicles. Plays a role in the lysosomal trafficking of CTSD/cathepsin D from the Golgi to lysosomes. Also promotes the recycling of transferrin directly from early endosomes to the plasma membrane. Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate (PtdInsP3).

Protein type: C2H2-type zinc finger protein; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 3p25.1

Cellular Component: intracellular membrane-bound organelle; early endosome membrane; plasma membrane; endosome membrane; endosome

Molecular Function: protein binding; zinc ion binding; Rab GTPase binding

Biological Process: protein transport; late endosome to vacuole transport; endosomal vesicle fusion; Golgi to endosome transport; Golgi to vacuole transport; endocytosis; blood coagulation; endosome transport; vacuole inheritance