Rabbit ZEB1 Polyclonal Antibody | anti-ZEB1 antibody

ZEB1 Rabbit Polyclonal

Cat. Num. AAA7605853

• Gene Names: ZEB1; BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: ZEB1; Polyclonal Antibody; ZEB1 Rabbit Polyclonal; anti-ZEB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 1036

• Applicable Applications for anti-ZEB1 antibody: ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
• Immunogen: Zinc finger E-box binding homeobox 1
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using MBS7605853 (ZEB1 Antibody) at dilution of 1:1000)

Western Blot (WB)

(MCF7 cells were subjected to SDS PAGE followed by western blot with MBS7605853 (ZEB1 antibody) at dilution of 1:500)

NCBI and Uniprot Product Information

• NCBI GI #: 1021312335
• NCBI GeneID: 6935
• NCBI Accession #: NP_001310604.1
• NCBI GenBank Nucleotide #: NM_001323675.1
• Molecular Weight: 122,084 Da
• NCBI Official Full Name: zinc finger E-box-binding homeobox 1 isoform i
• NCBI Official Synonym Full Names: zinc finger E-box binding homeobox 1
• NCBI Official Symbol: ZEB1
• NCBI Official Synonym Symbols: BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1
• NCBI Protein Information: zinc finger E-box-binding homeobox 1
• UniProt Protein Name: Zinc finger E-box-binding homeobox 1
• Protein Family: Zinc finger E-box-binding homeobox
• UniProt Gene Name: ZEB1
• UniProt Synonym Gene Names: AREB6; TCF8; TCF-8
• UniProt Entry Name: ZEB1_HUMAN

NCBI Description

This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2010]

Uniprot Description

TCF8: Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs. Interacts (via N-terminus) with SMARCA4/BRG1. Colocalizes with SMARCA4/BRG1 in E-cadherin- negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas. Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas. Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.

Protein type: Transcription, coactivator/corepressor; Motility/polarity/chemotaxis; C2H2-type zinc finger protein; DNA-binding

Chromosomal Location of Human Ortholog: 10p11.2

Cellular Component: cytoplasm; nucleoplasm; nucleus; transcription factor complex

Molecular Function: chromatin binding; protein binding; transcription coactivator activity; transcription corepressor activity; transcription factor activity; transcription factor binding; zinc ion binding

Biological Process: cartilage development; cell proliferation; central nervous system development; embryonic camera-type eye morphogenesis; embryonic skeletal morphogenesis; immune response; negative regulation of cell proliferation; negative regulation of epithelial cell differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; pattern specification process; positive regulation of neuron differentiation; positive regulation of transcription from RNA polymerase II promoter; regulation of mesenchymal cell proliferation; regulation of smooth muscle cell differentiation; regulation of T cell differentiation in the thymus; regulation of transcription from RNA polymerase II promoter; regulation of transforming growth factor beta receptor signaling pathway; semicircular canal morphogenesis; transcription, DNA-dependent

Disease: Corneal Dystrophy, Fuchs Endothelial, 6; Corneal Dystrophy, Posterior Polymorphous, 3