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Western Blot (WB) (Western blot analysis of ZDHHC9 Antibody (C-term) in mouse liver tissue lysates (35ug/lane). ZDHHC9 (arrow) was detected using the purified Pab.)

Rabbit anti-Human, Mouse ZDHHC9 Polyclonal Antibody | anti-ZDHHC9 antibody

ZDHHC9, CT (ZDHHC9, CXorf11, ZDHHC10, ZNF379, ZNF380, Palmitoyltransferase ZDHHC9, Zinc finger DHHC domain-containing protein 9, Zinc finger protein 379, Zinc finger protein 380) (Biotin)

Gene Names
ZDHHC9; CGI89; DHHC9; MMSA1; MRXSZ; ZNF379; ZNF380; CXorf11; ZDHHC10
Reactivity
Human, Mouse
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ZDHHC9; Polyclonal Antibody; CT (ZDHHC9; CXorf11; ZDHHC10; ZNF379; ZNF380; Palmitoyltransferase ZDHHC9; Zinc finger DHHC domain-containing protein 9; Zinc finger protein 379; Zinc finger protein 380) (Biotin); Zinc finger protein 380; anti-ZDHHC9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-ZDHHC9 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
ZDHHC9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 335~364 amino acids from the C-terminal region of human ZDHHC9.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ZDHHC9 Antibody (C-term) in mouse liver tissue lysates (35ug/lane). ZDHHC9 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of ZDHHC9 Antibody (C-term) in mouse liver tissue lysates (35ug/lane). ZDHHC9 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(ZDHHC9 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZDHHC9 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (ZDHHC9 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZDHHC9 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-ZDHHC9 antibody
The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS.
Product Categories/Family for anti-ZDHHC9 antibody
References
Mansilla, F., et al. Br. J. Cancer 96(12):1896-1903(2007). Raymond, F.L., et al. Am. J. Hum. Genet. 80(5):982-987(2007). Zhou, F.L., et al. Cancer Immunol. Immunother. 55(8):910-917(2006).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40,916 Da
NCBI Official Full Name
palmitoyltransferase ZDHHC9
NCBI Official Synonym Full Names
zinc finger, DHHC-type containing 9
NCBI Official Symbol
ZDHHC9
NCBI Official Synonym Symbols
CGI89; DHHC9; MMSA1; MRXSZ; ZNF379; ZNF380; CXorf11; ZDHHC10
NCBI Protein Information
palmitoyltransferase ZDHHC9; antigen MMSA-1; zinc finger protein 379; zinc finger protein 380; zinc finger, DHHC domain containing 10; Asp-His-His-Cys domain containing protein 9
UniProt Protein Name
Palmitoyltransferase ZDHHC9
Protein Family
UniProt Gene Name
ZDHHC9
UniProt Synonym Gene Names
CXorf11; ZDHHC10; ZNF379; ZNF380; DHHC-9; DHHC9
UniProt Entry Name
ZDHC9_HUMAN

NCBI Description

This gene encodes an integral membrane protein that is a member of the zinc finger DHHC domain-containing protein family. The encoded protein forms a complex with golgin subfamily A member 7 and functions as a palmitoyltransferase. This protein specifically palmitoylates HRAS and NRAS. Mutations in this gene are associated with X-linked mental retardation. Alternate splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, May 2010]

Uniprot Description

ZDHHC9: The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS. Defects in ZDHHC9 are the cause of mental retardation syndromic X-linked ZDHHC9-related (MRXSZ). A disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Some patients have marfanoid habitus as an additional feature. Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily.

Protein type: EC 2.3.1.225; Membrane protein, multi-pass; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: Xq26.1

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; endoplasmic reticulum; cytoplasm; intrinsic to Golgi membrane; integral to membrane

Molecular Function: zinc ion binding; Ras palmitoyltransferase activity; palmitoyltransferase activity; protein-cysteine S-palmitoleyltransferase activity

Biological Process: peptidyl-S-palmitoyl-L-cysteine biosynthetic process from peptidyl-cysteine; protein palmitoylation

Disease: Mental Retardation, X-linked, Syndromic, Raymond Type

Research Articles on ZDHHC9

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Product Notes

The ZDHHC9 zdhhc9 (Catalog #AAA6365086) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZDHHC9, CT (ZDHHC9, CXorf11, ZDHHC10, ZNF379, ZNF380, Palmitoyltransferase ZDHHC9, Zinc finger DHHC domain-containing protein 9, Zinc finger protein 379, Zinc finger protein 380) (Biotin) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ZDHHC9 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-100 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ZDHHC9 zdhhc9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZDHHC9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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