Western Blot (WB)
(Western blot analysis of lysate from SH-SY5Y cell line, using ZDHHC21 Antibody (N-term)(MBS9204336). MBS9204336 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Rabbit ZDHHC21 Polyclonal Antibody | anti-ZDHHC21 antibody
ZDHHC21 Antibody (N-term)
Western Blot (WB)
(Western blot analysis of lysate from SH-SY5Y cell line, using ZDHHC21 Antibody (N-term)(MBS9204336). MBS9204336 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Western Blot (WB)
(Western blot analysis of lysate from SH-SY5Y cell line, using ZDHHC21 Antibody (N-term)(MBS9204336). MBS9204336 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Western Blot (WB)
(Western blot analysis of ZDHHC21 Antibody (N-term) in mouse liver tissue lysates (35ug/lane).ZDHHC21 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of ZDHHC21 Antibody (N-term) in mouse liver tissue lysates (35ug/lane).ZDHHC21 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(ZDHHC21 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZDHHC21 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(ZDHHC21 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZDHHC21 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(ZDHHC21 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(ZDHHC21 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Le Clerc, S., et al. J. Infect. Dis. 200(8):1194-1201(2009)
Humphray, S.J., et al. Nature 429(6990):369-374(2004)
NCBI and Uniprot Product Information
Uniprot Description
ZDHHC21: Palmitoylates sex steroid hormone receptors, including ESR1, PGR and AR, thereby regulating their targeting to the plasma membrane. This affects rapid intracellular signaling by sex hormones via ERK and AKT kinases and the generation of cAMP, but does not affect that mediated by their nuclear receptor. Belongs to the DHHC palmitoyltransferase family.
Protein type: EC 2.3.1.225; Membrane protein, multi-pass; Membrane protein, integral; Transferase
Chromosomal Location of Human Ortholog: 9p22.3
Cellular Component: Golgi membrane; Golgi apparatus; integral to membrane; plasma membrane
Molecular Function: zinc ion binding; palmitoyltransferase activity; protein-cysteine S-palmitoleyltransferase activity
Biological Process: peptidyl-S-palmitoyl-L-cysteine biosynthetic process from peptidyl-cysteine; protein palmitoylation; hair follicle development; sebaceous gland development; regulation of nitric-oxide synthase activity; nitric oxide metabolic process
Research Articles on ZDHHC21
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Product Notes
The ZDHHC21 zdhhc21 (Catalog #AAA9204336) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 59-87. The ZDHHC21 Antibody (N-term) reacts with Human, mouse (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's ZDHHC21 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the ZDHHC21 zdhhc21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZDHHC21, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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