Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ZC3H12A antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Rabbit anti-Human ZC3H12A Polyclonal Antibody | anti-ZC3H12A antibody
ZC3H12A Polyclonal Antibody
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ZC3H12A antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ZC3H12A antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
NCBI and Uniprot Product Information
NCBI Description
ZC3H12A is an MCP1 (CCL2; MIM 158105)-induced protein that acts as a transcriptional activator and causes cell death of cardiomyocytes, possibly via induction of genes associated with apoptosis.[supplied by OMIM, Mar 2008]
Uniprot Description
MCPIP1: Has RNase activity and selectively degrades specific target mRNA species. Modulates the immune response and inflammation by regulating the decay of specific mRNA molecules. Recognizes the 3'-untranslated region (UTR) of the mRNA for IL6, CALCR and IL12B. Required for normal decay of IL6 mRNA. Triggers apoptosis and promotes angiogenesis in response to the binding of CCL2 to CCR2. Regulates expression of CDH12 and CHD19. Belongs to the ZC3H12 family.
Protein type: EC 3.1.-.-; Hydrolase
Chromosomal Location of Human Ortholog: 1p34.3
Cellular Component: cytoplasm; cytoskeleton; nucleoplasm; nucleus; protein complex; rough endoplasmic reticulum
Molecular Function: chromatin binding; DNA binding; endoribonuclease activity; exoribonuclease activity; miRNA binding; mRNA 3'-UTR binding; mRNA binding; protein binding; ribonuclease activity; ribosome binding; RNA binding; ubiquitin-specific protease activity
Biological Process: cellular response to glucose starvation; inhibition of NF-kappaB transcription factor; mRNA catabolic process, endonucleolytic cleavage-dependent decay; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of interleukin-1 beta secretion; negative regulation of interleukin-6 production; negative regulation of macrophage activation; negative regulation of NF-kappaB import into nucleus; negative regulation of nitric oxide biosynthetic process; negative regulation of protein amino acid phosphorylation; negative regulation of tumor necrosis factor production; positive regulation of angiogenesis; positive regulation of autophagy; positive regulation of defense response to virus by host; positive regulation of fat cell differentiation; positive regulation of protein import into nucleus; positive regulation of transcription from RNA polymerase II promoter; protein deubiquitination; protein oligomerization; regulation of gene expression; response to DNA damage stimulus
Research Articles on ZC3H12A
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Product Notes
The ZC3H12A zc3h12a (Catalog #AAA9132542) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZC3H12A Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZC3H12A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000 - 1:3000. Researchers should empirically determine the suitability of the ZC3H12A zc3h12a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZC3H12A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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