Rabbit anti-Human, Mouse ZBTB7B Polyclonal Antibody | anti-ZBTB7B antibody

ZBTB7B Antibody

Cat. Num. AAA9631010

• Gene Names: ZBTB7B; CKROX; THPOK; ZFP67; ZBTB15; ZFP-67; c-KROX; hcKROX; ZNF857B
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: ZBTB7B; Polyclonal Antibody; ZBTB7B Antibody; c Krox; cKrox; DKFZp686G01254; hc Krox; hcKrox; Krueppel-related zinc finger protein cKrox; Kruppel related zinc finger protein c Krox; Kruppel related zinc finger protein cKrox; Kruppel related zinc finger protein hcKrox; T helper inducing POZ/Kruppel like factor; T-helper-inducing POZ/Krueppel-like factor; Th-POK; ThPOK; ZBT7B_HUMAN; ZBTB 15; ZBTB 7B; ZBTB15; ZFP 67; Zfp-67; ZFP67; Zinc finger and BTB domain containing 15; Zinc finger and BTB domain containing 7B; Zinc finger and BTB domain containing protein 15; Zinc finger and BTB domain containing protein 7B; Zinc finger and BTB domain-containing protein 15; Zinc finger and BTB domain-containing protein 7B; Zinc finger protein 67 homolog; Zinc finger protein 857B; Zinc finger protein Th POK; Zinc finger protein Th-POK; Zinc finger protein ThPOK; ZNF857B; anti-ZBTB7B antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: ZBTB7B Antibody detects endogenous levels of ZBTB7B.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-ZBTB7B antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human ZBTB7B.
• Conjugation: Unconjugated
• Post Translational Modifications: Acetylated directly and specifically by EP300. EP300-mediated acetylation of Lys-206, Lys-212 and Lys-335 stabilizes the protein by antagonizing ubiquitin conjugation (By similarity).Ubiquitinated, leading to proteasomal degradation. Competes with acetylation on Lys-206, Lys-212 and Lys-335 (By similarity).
• Subunit Structure: Homodimerizes. Interacts with NCL, NEDD4 and YBX1. Interacts with HNRNPU (via RNA-binding RGG-box region); the interaction facilitates the recruitment of long non-coding RNA Blnc1 by ZBTB7B. Interacts with HDAC4 and HDAC5; the interaction allows the recruitment of HDAC4 and HDAC5 on CD8 loci for deacetylation and possible inhibition of CD8 genes expression.
• Function: Transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. Exerts distinct biological functions in the mammary epithelial cells and T cells in a tissue-specific manner. Necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. Cross-antagonism between ZBTB7B and CBF complexes are determinative to CD4 versus CD8 cell fate decision. Suppresses RUNX3 expression and imposes CD4+ lineage fate by inducing the SOCS suppressors of cytokine signaling. induces, as a transcriptional activator, SOCS genes expression which represses RUNX3 expression and promotes the CD4+ lineage fate. During CD4 lineage commitment, associates with multiple sites at the CD8 locus, acting as a negative regulator of the CD8 promoter and enhancers by epigenetic silencing through the recruitment of class II histone deacetylases, such as HDAC4 and HDAC5, to these loci. Regulates the development of IL17-producing CD1d-restricted naural killer (NK) T cells. Also functions as an important metabolic regulator in the lactating mammary glands. Critical feed-forward regulator of insulin signaling in mammary gland lactation, directly regulates expression of insulin receptor substrate-1 (IRS-1) and insulin-induced Akt-mTOR-SREBP signaling (By similarity). Transcriptional repressor of the collagen COL1A1 and COL1A2 genes. May also function as a repressor of fibronectin and possibly other extracellular matrix genes. Potent driver of brown fat development, thermogenesis and cold-induced beige fat formation. Recruits the brown fat lncRNA 1 (Blnc1):HNRNPU ribonucleoprotein complex to activate thermogenic gene expression in brown and beige adipocytes (By similarity).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-ZBTB7B antibody

Nucleus; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 51316943
• NCBI GeneID: 51043
• UniProt Accession #: O15156; Q68DR2; Q96EP2; B4E3K5; D3DV83; J3KQQ3
• Molecular Weight: 539
• NCBI Official Full Name: Zinc finger and BTB domain-containing protein 7B
• NCBI Official Synonym Full Names: zinc finger and BTB domain containing 7B
• NCBI Official Symbol: ZBTB7B
• NCBI Official Synonym Symbols: CKROX; THPOK; ZFP67; ZBTB15; ZFP-67; c-KROX; hcKROX; ZNF857B
• NCBI Protein Information: zinc finger and BTB domain-containing protein 7B; zinc finger protein 857B; zinc finger protein Th-POK; zinc finger protein 67 homolog; zinc finger and BTB domain containing 15; T-helper-inducing POZ/Krueppel-like factor; krueppel-related zinc finger prot
• UniProt Protein Name: Zinc finger and BTB domain-containing protein 7B
• Protein Family: Zinc finger and BTB domain-containing protein
• UniProt Gene Name: ZBTB7B
• UniProt Synonym Gene Names: ZBTB15; ZFP67; ZNF857B; hcKrox; Zfp-67
• UniProt Entry Name: ZBT7B_HUMAN

NCBI Description

This gene encodes a zinc finger-containing transcription factor that acts as a key regulator of lineage commitment of immature T-cell precursors. It is necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. It also functions as a transcriptional repressor of type I collagen genes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

ZBTB7B: Transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. Necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. Transcriptional repressor of the collagen COL1A1 and COL1A2 genes. May also function as a repressor of fibronectin and possibly other extracellular matrix genes.

Protein type: C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: nucleoplasm; nucleus

Molecular Function: DNA binding; metal ion binding

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; multicellular organismal development; ectoderm development; cell differentiation