Western Blot (WB)
(ZBTB7A Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ZBTB7A antibody detected the ZBTB7A protein (arrow).)
Rabbit anti-Human ZBTB7A Polyclonal Antibody | anti-ZBTB7A antibody
ZBTB7A, CT (ZBTB7A, FBI1, LRF, ZBTB7, ZNF857A, Zinc finger and BTB domain-containing protein 7A, Factor binding IST protein 1, Factor that binds to inducer of short transcripts protein 1, HIV-1 1st-binding protein 1, Leukemia/lymphoma-related factor, POZ
Gene Names
ZBTB7A; LRF; FBI1; FBI-1; ZBTB7; ZNF857A; pokemon
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ZBTB7A; Polyclonal Antibody; CT (ZBTB7A; FBI1; LRF; ZBTB7; ZNF857A; Zinc finger and BTB domain-containing protein 7A; Factor binding IST protein 1; Factor that binds to inducer of short transcripts protein 1; HIV-1 1st-binding protein 1; Leukemia/lymphoma-related factor; POZ; POZ and Krueppel erythroid myeloid ontogenic factor; TTF-I-interacting peptide 21; Zinc finger protein 857A; anti-ZBTB7A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-ZBTB7A antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
ZBTB7A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 513-542 amino acids from the C-terminal region of human ZBTB7A.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(ZBTB7A Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ZBTB7A antibody detected the ZBTB7A protein (arrow).)
Western Blot (WB)
(ZBTB7A Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ZBTB7A antibody detected the ZBTB7A protein (arrow).)
Immunohistochemistry (IHC)
(ZBTB7A Antibody (C-term) (AP14208b)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ZBTB7A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(ZBTB7A Antibody (C-term) (AP14208b)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ZBTB7A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-ZBTB7A antibody
ZBTB7A plays a key role in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals (By similarity). Specifically represses the transcription of the CDKN2A gene. Efficiently abrogates E2F1-dependent CDKN2A transactivation/de-repression. Binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3' (By similarity).
Product Categories/Family for anti-ZBTB7A antibody
References
Qu, H., et al. Cancer Invest. 28(6):672-678(2010). Zu, X., et al. Cell. Mol. Biol. Lett. 15(2):260-271(2010). Cui, J., et al. Mol. Biol. Rep. 37(3):1627-1632(2010). Choi, W.I., et al. J. Biol. Chem. 284(19):12633-12644(2009). Choi, W.I., et al. Cell. Physiol. Biochem. 23 (4-6), 359-370 (2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61,439 Da
NCBI Official Full Name
zinc finger and BTB domain-containing protein 7A
NCBI Official Synonym Full Names
zinc finger and BTB domain containing 7A
NCBI Official Symbol
ZBTB7A
NCBI Official Synonym Symbols
LRF; FBI1; FBI-1; ZBTB7; ZNF857A; pokemon
NCBI Protein Information
zinc finger and BTB domain-containing protein 7A; TIP21; lymphoma related factor; zinc finger protein 857A; HIV-1 1st-binding protein 1; TTF-I-interacting peptide 21; factor binding IST protein 1; leukemia/lymphoma-related factor; POK erythroid myeloid on
UniProt Protein Name
Zinc finger and BTB domain-containing protein 7A
Protein Family
UniProt Gene Name
ZBTB7A
UniProt Synonym Gene Names
FBI1; LRF; ZBTB7; ZNF857A; FBI-1; POK erythroid myeloid ontogenic factor; Pokemon; TIP21
UniProt Entry Name
ZBT7A_HUMAN
Uniprot Description
FBI1: a widely expressed zinc finger protein. Apparently interacts with Bcl-6.
Protein type: DNA-binding; Transcription factor; C2H2-type zinc finger protein
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: nucleus
Molecular Function: histone acetyltransferase binding; protein binding; DNA binding; metal ion binding
Biological Process: transcription, DNA-dependent; multicellular organismal development; negative regulation of transcription from RNA polymerase II promoter; cell differentiation; negative regulation of transcription, DNA-dependent
Research Articles on ZBTB7A
Similar Products
Product Notes
The ZBTB7A zbtb7a (Catalog #AAA6364734) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZBTB7A, CT (ZBTB7A, FBI1, LRF, ZBTB7, ZNF857A, Zinc finger and BTB domain-containing protein 7A, Factor binding IST protein 1, Factor that binds to inducer of short transcripts protein 1, HIV-1 1st-binding protein 1, Leukemia/lymphoma-related factor, POZ reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZBTB7A can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:10-50 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ZBTB7A zbtb7a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZBTB7A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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