Rabbit anti-Human ZBED3 Polyclonal Antibody | anti-ZBED3 antibody

ZBED3 Antibody

Cat. Num. AAA151505

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: ZBED3 Antibody is affinity chromatography purified via peptide column.
• Synonyms: ZBED3; Polyclonal Antibody; ZBED3 Antibody; Zinc finger BED domain-containing protein 3; Axin-interacting protein; zinc finger; BED-type containing 3; anti-ZBED3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody is predicted to have no cross-reactivity to ZBED1 or ZBED2.
• Purity/Purification: ZBED3 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 234

• Applicable Applications for anti-ZBED3 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: ZBED3 antibody can be used for detection of ZBED3 by Western blot at 1 mug/mL. Despite its predicted molecular weight, ZBED3 often migrates at a higher position in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: ZBED3 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human ZBED3.
• Buffer: ZBED3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: ZBED3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of ZBED3 in Jurkat cell lysate with ZBED3 antibody at 1 μg/ml.)

Immunocytochemistry (IHC)

(Immunocytochemistry of ZBED3 in A549 cells with ZBED3 antibody at 20 μg/mL.)

Related Product Information for anti-ZBED3 antibody

ZBED3 Antibody: Axin is a key modulator of the Wnt/beta-catenin pathway and acts as a scaffold protein in phosphorylating and degrading cytoplasmic beta-catenin. ZBED3, a member of the Zinc finger BED-type domain containing superfamily, has been recently identified as an Axin-interacting protein and has been suggested to modulate the Wnt/beta-catenin signaling pathway. RNA interference (RNAi) experiments targeting ZBED3 RNA attenuated Wnt-induced beta-catenin accumulation, lymphoid enhancer binding factor-1-dependent luciferase reporter activity, and Wnt gene expression.

NCBI and Uniprot Product Information

• NCBI GI #: 14150185
• NCBI GeneID: 84327
• NCBI Accession #: NP_115743
• NCBI GenBank Nucleotide #: NM_032367.2
• UniProt Accession #: Q96IU2
• Molecular Weight: 25,132 Da
• NCBI Official Full Name: zinc finger BED domain-containing protein 3
• NCBI Official Synonym Full Names: zinc finger, BED-type containing 3
• NCBI Official Symbol: ZBED3
• NCBI Protein Information: zinc finger BED domain-containing protein 3; 2610005H11Rik; axin-interacting protein; zinc finger, BED domain containing 3
• UniProt Protein Name: Zinc finger BED domain-containing protein 3
• UniProt Gene Name: ZBED3
• UniProt Entry Name: ZBED3_HUMAN

Uniprot Description

ZBED3: Acts as a positive regulator in the activation of the canonical Wnt/beta-catenin signaling pathway by stabilizing cytoplasmic beta-catenin. Involved in transcription activation of Wnt target gene expression. Interacts with AXIN1; the interaction is direct, enhanced by protein kinase GSK3B and casein kinase CSNK1E activities and decreases GSK3B-induced beta-catenin serine and threonine phosphorylations

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 5q13.3

Cellular Component: membrane; cytosol

Molecular Function: DNA binding; metal ion binding

Biological Process: Wnt receptor signaling pathway; negative regulation of protein amino acid phosphorylation; protein stabilization; positive regulation of transcription from RNA polymerase II promoter