Rabbit ZAP70 Polyclonal Antibody | anti-phospho-ZAP70 antibody

Anti-phospho-ZAP70 (Tyr319)

Cat. Num. AAA516304

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity chromatography
• Synonyms: ZAP70; Polyclonal Antibody; Anti-phospho-ZAP70 (Tyr319); anti-phospho-ZAP70 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit, IgG
• Specificity: Recognizes the ZAP70 protein phosphorylated at tyrosine 319
• Purity/Purification: Affinity chromatography
• Form/Format: Phosphate buffered saline (without Mg2+ and Ca2+), pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Applicable Applications for anti-phospho-ZAP70 antibody: Western Blot (WB)
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human ZAP-70 around the phosphorylation site of tyrosine 319 (S-P-YP-S-D).
• Preparation and Storage: Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.; 1 year at -20 degree C from date of shipment

Western Blot (WB)

(Sample Data: Western blot analysis of extract from Jurkat cells using ZAP70 antibody (Lane 1 and 2) and Anti-phospho-ZAP70 (Tyr319) (Lane 3 and 4).)

Related Product Information for anti-phospho-ZAP70 antibody

ZAP70 is a non-receptor protein tyrosine kinase (part of the Syk/Zap70 family) that is involved in signaling by the T-cell antigen receptor (TCR). Ligation of the TCR/CD3 receptor in Jurkat T-cells induces phosphoprotein complexes which contain ZAP70 (1). TCR zeta chains are initially phosphorylated by p56Lck that lead to the recruitment of ZAP70 via its SH2 domain. ZAP70 in turn phosphorylates other proteins in the TCR-phosphoprotein complex. One of the natural substrates for ZAP70 is the zeta-chain dimer of the TCR/CD3 complex (2).

Product Categories/Family for anti-phospho-ZAP70 antibody

Phospho Specific Antibodies; Cancer; Cytoplasmic Tyrosine Kinases; Inflammation; NfkB Pathway

References

1. Duplay, P. et al: p56lck interacts via its src homology 2 domain with the ZAP-70 kinase. J Exp Med. 1994 Apr 1;179(4):1163-72.
2. Chan, A C. et al: Differential expression of ZAP-70 and Syk protein tyrosine kinases, and the role of this family of protein tyrosine kinases in TCR signaling. J Immunol. 1994 May 15;152(10):4758-66.
3. Salomon AR, et al. (2003) Proc Natl Acad Sci U S A; 100(2): 443-448
4. Ku GM, et al. (2001) EMBO J; 20(3): 457-465.
5. Tang J, et al. (1999) Proc Natl Acad Sci U S A; 96(17): 9775-9780.
6. Zhao Q, et al. (1996) Mol Cell Biol; 16(12): 6765-6774. 7. Williams BL, et al. (1999) EMBO J; 18(7): 1832-1844.