Western Blot (WB)
(Western blot analysis of ZAK (Phospho-Ser165) using HeLa whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Rabbit ZAK Polyclonal Antibody | anti-ZAK antibody
Phospho-ZAK (Ser165) Antibody
Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (88%)
Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (88%)
Tissue Specificity: Ubiquitously expressed. Isoform 2 is the predominant form in all tissues examined, except for liver, in which isoform 1 is more highly expressed.
IF/ICC: 1:100-1:500
Peptide ELISA: 1:20,000-1:40,000
Note: Translocates to the nucleus upon ultraviolet B irradiation.
Western Blot (WB)
(Western blot analysis of ZAK (Phospho-Ser165) using HeLa whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Western Blot (WB)
(Western blot analysis of ZAK (Phospho-Ser165) using HeLa whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Staining A549 cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(AF8195) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Staining A549 cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(AF8195) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Uniprot Description
ZAK: a mixed-lineage kinase containing a leucine-zipper (LZ) and a sterile-alpha motif (SAM). It is reported to activate the JNK/SAPK and NF-kappaB pathways.
Protein type: EC 2.7.11.25; Kinase, protein; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); TKL group; MLK family; MLK subfamily
Chromosomal Location of Human Ortholog: 2q24.2
Cellular Component: cytoplasm; nucleus
Molecular Function: protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase activity; magnesium ion binding; ATP binding
Biological Process: cell death; cell proliferation; response to radiation; activation of MAPKK activity; positive regulation of apoptosis; DNA damage checkpoint; response to stress; cytoskeleton organization and biogenesis; cell cycle arrest; cell differentiation; protein amino acid phosphorylation; cell cycle checkpoint; activation of JNK activity
Research Articles on ZAK
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Product Notes
The ZAK mltk (Catalog #AAA9614196) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-ZAK (Ser165) Antibody reacts with Human, Mouse Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (88%) and may cross-react with other species as described in the data sheet. AAA Biotech's ZAK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA). WB: 1:1000-3000 IF/ICC: 1:100-1:500 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the ZAK mltk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZAK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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