Rabbit Yotiao Polyclonal Antibody | anti-AKAP9 antibody

Yotiao (AKAP9, PRKA9, CG-NAP)

Cat. Num. AAA616848

• Gene Names: AKAP9; PRKA9; AKAP-9; CG-NAP; YOTIAO; AKAP350; AKAP450; HYPERION; KIAA0803; MU-RMS-40.16A
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Yotiao; Polyclonal Antibody; Yotiao (AKAP9; PRKA9; CG-NAP); Anti -Yotiao (AKAP9; anti-AKAP9 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Yotiao protein. Species Crossreactivity: mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.

• Applicable Applications for anti-AKAP9 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 0.1-1.0ug/ml; Western Blot: 1-3ug/ml; Fetal mouse brain~230kD; AsPC-1 lysates and rat brain homogenates, multiple bands at other molecular weights have also been observed.
• Immunogen: Synthetic peptide derived from the C-terminal region of the human Yotiao protein.
• Positive Control: AsPC-1 cell lysates, rat and fetal mouse brain tissue homogenates.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-AKAP9 antibody

Yotiao is a novel cytoskeletal protein specifically concentrated in the neuromuscular junction and neuronal synapes that interacts with specific splice variants of NMDA Receptor Subunit NR1 in a C1 exon cassette-dependent manner (1). Yotiao has also been shown to physically attach the type I protein phosphatase (PP1) and the adenosine 3',5'-mono-phosphate (cAMP)- dependent protein kinase (PKA) holoenzyme to NMDA receptors to regulate channel activity (2). NMDA receptors are involved with many important functions and dysfunctions of the nervous system, including synapse formation, synaptic plasticity, and excitotoxicity. Yotiao research may lead to a clearer understanding of these neural processes by explaining how NMDA receptors are targeted to particular synapses, how this localization is regulated during development and synaptic activity, and how NMDA receptor activity is transduced into intracellular signals responsible for particular neuronal responses (1). Human tissue distribution studies with yotiao mRNA revealed that yotiao is abundantly present in skeletal muscle and pancreas, with lower expression in heart, placenta, and brain. Yotiao mRNA was undetectable in lung, liver, and kidney (1).

Product Categories/Family for anti-AKAP9 antibody

Antibodies; Abs to Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 2623068
• NCBI GeneID: 10142
• UniProt Accession #: Q5GIA7; Q5IBP5; Q6PJH3; Q8IW64; Q96KG3; Q99996; Q9UFL2
• Molecular Weight: 118,199 Da
• NCBI Official Full Name: yotiao
• NCBI Official Synonym Full Names: A kinase (PRKA) anchor protein (yotiao) 9
• NCBI Official Symbol: AKAP9
• NCBI Official Synonym Symbols: PRKA9; AKAP-9; CG-NAP; YOTIAO; AKAP350; AKAP450; HYPERION; KIAA0803; MU-RMS-40.16A
• NCBI Protein Information: A-kinase anchor protein 9; protein yotiao; protein hyperion; OTTHUMP00000161771; AKAP 120-like protein; AKAP9-BRAF fusion protein; kinase N-associated protein; A-kinase anchor protein 350 kDa; A-kinase anchor protein 450 kDa; protein kinase A anchoring protein 9; centrosome- and Golgi-localized PKN-associated protein
• UniProt Protein Name: Antigen MU-RMS-40.16A
• Protein Family: A-kinase anchor protein
• UniProt Entry Name: Q5GIA7_HUMAN

NCBI Description

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternate splicing of this gene results in at least two isoforms that localize to the centrosome and the Golgi apparatus, and interact with numerous signaling proteins from multiple signal transduction pathways. These signaling proteins include type II protein kinase A, serine/threonine kinase protein kinase N, protein phosphatase 1, protein phosphatase 2a, protein kinase C-epsilon and phosphodiesterase 4D3. [provided by RefSeq]

Uniprot Description

AKAP9: Binds to type II regulatory subunits of protein kinase A. Scaffolding protein that assembles several protein kinases and phosphatases on the centrosome and Golgi apparatus. May be required to maintain the integrity of the Golgi apparatus. Isoform 4 is associated with the N-methyl-D-aspartate receptor and is specifically found in the neuromuscular junction (NMJ) as well as in neuronal synapses, suggesting a role in the organization of postsynaptic specializations. Defects in AKAP9 are the cause of long QT syndrome type 11 (LQT11). Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. They can present with a sentinel event of sudden cardiac death in infancy. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 7q21-q22

Cellular Component: Golgi apparatus; centrosome; pericentriolar material; voltage-gated potassium channel complex; cytoskeleton; intracellular membrane-bound organelle; cytosol

Molecular Function: protein binding; potassium channel regulator activity; protein complex scaffold; receptor binding

Biological Process: synaptic transmission; transport; organelle organization and biogenesis; spermatogenesis; mitotic cell cycle; signal transduction; G2/M transition of mitotic cell cycle; positive regulation of peptidyl-serine phosphorylation; Sertoli cell development

Disease: Long Qt Syndrome 11