Rabbit anti-Human YOD1 Polyclonal Antibody | anti-YOD1 antibody

YOD1, CT (YOD1, DUBA8, HIN7, OTUD2, Ubiquitin thioesterase OTU1, DUBA-8, HIV-1-induced protease 7, OTU domain-containing protein 2) (FITC)

Cat. Num. AAA6364339

• Gene Names: YOD1; DUBA8; OTUD2; PRO0907
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: YOD1; Polyclonal Antibody; CT (YOD1; DUBA8; HIN7; OTUD2; Ubiquitin thioesterase OTU1; DUBA-8; HIV-1-induced protease 7; OTU domain-containing protein 2) (FITC); OTU domain-containing protein 2; anti-YOD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-YOD1 antibody: Immunofluorescence (IF), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: YOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 326-355 amino acids from the C-terminal region of human YOD1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(YOD1 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the YOD1 antibody detected the YOD1 protein (arrow).)

Immunohistochemistry (IHC)

(YOD1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of YOD1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of YOD1 Antibody (C-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-YOD1 antibody

Deubiquitinating enzymes (DUBs; see MIM 603478) are proteases that specifically cleave ubiquitin (MIM 191339) linkages, negating the action of ubiquitin ligases. DUBA8 belongs to a DUB subfamily characterized by an ovarian tumor (OTU) domain.[supplied by OMIM].

Product Categories/Family for anti-YOD1 antibody

Antibodies; Ubiquitin

References

Ernst, R., et al. Mol. Cell 36(1):28-38(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 62751964
• NCBI GeneID: 55432
• NCBI Accession #: NP_061036.3
• NCBI GenBank Nucleotide #: NM_018566.3
• UniProt Accession #: Q5VVQ6
• NCBI Official Full Name: ubiquitin thioesterase OTU1 isoform 1
• NCBI Official Synonym Full Names: YOD1 deubiquitinase
• NCBI Official Symbol: YOD1
• NCBI Official Synonym Symbols: DUBA8; OTUD2; PRO0907
• NCBI Protein Information: ubiquitin thioesterase OTU1
• UniProt Protein Name: Ubiquitin thioesterase OTU1
• UniProt Gene Name: YOD1
• UniProt Synonym Gene Names: DUBA8; HIN7; OTUD2; HIN-7; HsHIN7
• UniProt Entry Name: OTU1_HUMAN

NCBI Description

Protein ubiquitination controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. This dynamic process, involving ubiquitin conjugating enzymes and deubiquitinating enzymes, adds and removes ubiquitin. Deubiquitinating enzymes are cysteine proteases that specifically cleave ubiquitin from ubiquitin-conjugated protein substrates. The protein encoded by this gene belongs to a DUB subfamily characterized by an ovarian tumor (OTU) domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]

Uniprot Description

YOD1: Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Able to cleave both polyubiquitin and di- ubiquitin. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Lipid Metabolism - unsaturated fatty acid biosynthesis; Ubiquitin-specific protease; C2H2-type zinc finger protein; EC 3.4.19.12; Secondary Metabolites Metabolism - limonene and pinene degradation

Chromosomal Location of Human Ortholog: 1q32.2

Molecular Function: protein binding; metal ion binding; ubiquitin-specific protease activity

Biological Process: ER-associated protein catabolic process; unfolded protein response