Goat Ymer/CCDC50 Polyclonal Antibody | anti-CCDC50 antibody

Goat anti-Ymer/CCDC50 Antibody

Cat. Num. AAA420644

• Gene Names: CCDC50; YMER; C3orf6; DFNA44
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Rat, Dog, Cow
• Applications: ELISA, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: Ymer/CCDC50; Polyclonal Antibody; Goat anti-Ymer/CCDC50 Antibody; CCDC50; Ymer protein; coiled-coil domain containing 50; C3orf6; YMER; chromosome 3 open reading frame 6; DFNA44; Ymer protein antibody; coiled-coil domain containing 50 antibody; YMER antibody; C3orf6 antibody; chromosome 3 open reading frame 6 antibody; Ymer / CCDC50; anti-CCDC50 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Rat, Dog, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: CFSKSESSHKGFHYK
• Sequence Length: 482

• Applicable Applications for anti-CCDC50 antibody: Peptide ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Immunohistochemistry: In paraffin embedded Human Pancreas shows strong staining in cytoplasm in select acinar cells. Recommended concentration, 5-10ug/ml.; Western Blot: Preliminary experiments gave an approx 18kDa band in Human Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.3kDa according to NP_848018.1 and 35.8kDa according to NP_777568.1. The 18kDa band was successfully blocked by incubation with the immunizing peptide.
• Immunogen: Peptide with sequence CFSKSESSHKGFHYK, from the internal region of the protein sequence according to NP_848018.1; NP_777568.1.
• Epitope: Internal region
• Note: This antibody is expected to recognise both reported isoforms of human Ymer protein.
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

((5ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 41281911
• NCBI GeneID: 152137
• NCBI Accession #: NP_848018.1; NP_777568.1
• NCBI GenBank Nucleotide #: NM_178335.2
• Molecular Weight: 56,340 Da
• NCBI Official Full Name: coiled-coil domain-containing protein 50 long isoform
• NCBI Official Synonym Full Names: coiled-coil domain containing 50
• NCBI Official Symbol: CCDC50
• NCBI Official Synonym Symbols: YMER; C3orf6; DFNA44
• NCBI Protein Information: coiled-coil domain-containing protein 50
• UniProt Protein Name: Coiled-coil domain-containing protein 50
• Protein Family: Coiled-coil domain-containing protein
• UniProt Gene Name: CCDC50
• UniProt Synonym Gene Names: C3orf6
• UniProt Entry Name: CCD50_HUMAN

NCBI Description

This gene encodes a soluble, cytoplasmic, tyrosine-phosphorylated protein with multiple ubiquitin-interacting domains. Mutations in this gene cause nonsyndromic, postlingual, progressive sensorineural DFNA44 hearing loss. In mouse, the protein is expressed in the inner ear during development and postnatal maturation and associates with microtubule-based structures. This protein may also function as a negative regulator of NF-kB signaling and as an effector of epidermal growth factor (EGF)-mediated cell signaling. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Uniprot Description

Involved in EGFR signaling.