YARS antibody pair

YARS Antibody Pair

Cat. Num. AAA7041704

• Gene Names: YARS; YRS; YTS; TYRRS; CMTDIC
• Reactivity: Mouse, Pig, Dog
• Applications: ELISA
• Synonyms: YARS; Polyclonal Antibody Pair; YARS Antibody Pair; Tyrosyl-tRNA synthetase; cytoplasmic; Tyrosyl--tRNA ligase; TyrRS; YARS antibody pair

• Host: Capture: Rabbit; Detection: Rabbit
• Reactivity: Mouse, Pig, Dog
• Clonality: Polyclonal
• Isotype: Capture: IgG; Detection: IgG
• Form/Format: Liquid
• Concentration: The concentrations and volumes of the antibodies may vary and be lot specific. Details will be on the vial labels. (varies by lot)
• Sequence Length: 528

• Applicable Applications for YARS antibody pair: Sandwich ELISA (EIA)
• Application Notes: We recommend using the capture antibody at a concentration of 0.5ug/ml and the detection antibody at a concentration of 0.125ug/ml.
• Immunogen Species: Human
• Conjugation: Capture: Non-conjugated; Detection: Biotin conjugated
• Recommended Standard: K-562 cell; HL-60 cell; (Standards are recommendations only. Compatibility of the standards in the user developed application needs to be established and confirmed by the researcher.
• Storage Buffer: Capture: 50% Glycerol, 0.01M PBS, PH 7.4; Detection: 50% Glycerol, 0.01M PBS, PH 7.4
• Other Note: Immunogen information is company proprietary information. Antibodies are sold as a pair and not available separately.
• Preparation and Storage: Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze/thaw cycles. When stored properly, products should be stable for at least 5 months.

NCBI and Uniprot Product Information

• NCBI GI #: 823669220
• NCBI GeneID: 8565
• Molecular Weight: 59,143 Da
• NCBI Official Full Name: YARS, partial
• NCBI Official Synonym Full Names: tyrosyl-tRNA synthetase
• NCBI Official Symbol: YARS
• NCBI Official Synonym Symbols: YRS; YTS; TYRRS; CMTDIC
• NCBI Protein Information: tyrosine--tRNA ligase, cytoplasmic
• UniProt Protein Name: Tyrosine--tRNA ligase, cytoplasmic
• Protein Family: Tyrosine--tRNA ligase
• UniProt Gene Name: YARS
• UniProt Synonym Gene Names: TyrRS
• UniProt Entry Name: SYYC_HUMAN

NCBI Description

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine. [provided by RefSeq, Jul 2008]

Uniprot Description

YARS: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr). Defects in YARS are the cause of Charcot-Marie-Tooth disease dominant intermediate type C (CMTDIC). CMTDIC is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec. Belongs to the class-I aminoacyl-tRNA synthetase family.

Protein type: Ligase; EC 6.1.1.1

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: cytoplasm; cytosol; extracellular space; nucleus

Molecular Function: ATP binding; interleukin-8 receptor binding; protein binding; signal transducer activity; tRNA binding; tyrosine-tRNA ligase activity; valine-tRNA ligase activity

Biological Process: apoptosis; signal transduction; tRNA aminoacylation for protein translation; tyrosyl-tRNA aminoacylation; valyl-tRNA aminoacylation

Disease: Charcot-marie-tooth Disease, Dominant Intermediate C