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Rabbit YAFQ Polyclonal Antibody | anti-YAFQ antibody

Rabbit anti-Escherichia coli (strain K12) YAFQ Polyclonal Antibody

Gene Names
yafQ; ECK0226; JW0215
Reactivity
E Coli (strain K12)
Applications
ELISA, Western Blot
Purity
Protein A/G
Synonyms
YAFQ; Polyclonal Antibody; Rabbit anti-Escherichia coli (strain K12) YAFQ Polyclonal Antibody; YAFQ Antibody; mRNA interferase toxin YafQ; EC 3.1.-.-; Endoribonuclease YafQ; Toxin YafQ; yafQ b0225 JW0215; anti-YAFQ antibody
Ordering
Host
Rabbit
Reactivity
E Coli (strain K12)
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protein A/G
Form/Format
Liquid
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH7.4
Applicable Applications for anti-YAFQ antibody
ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
Immunogen
Recombinant Escherichia coli (strain K12) yafQ protein
Conjugation
Non-conjugate
Modification
Unmodified
Deliverables
1. 200ug recombinant immunogen protein/peptide (positive control).
2. 1mL preimmune serum (negative control)
3. 10mg rabbit polyclonal antibody purified by Protein A/G.
Quality Guarantee
1. Antibody purity >90% confirmed by SDS-PAGE.
2. Antibody titer > 1: 64,000 confirmed by ELISA
3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
Complementary Antibody
Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:
* 6*His Monoclonal Antibody #MBS1491670
* DYKDDDDK tag Monoclonal Antibody #MBS1493946
* GAPDH Monoclonal Antibody #MBS1495524
* GFP Monoclonal Antibody #MBS1496050
* Myc tag Monoclonal Antibody #MBS1497694
* GST Monoclonal Antibody #MBS1497871
* Sumo tag Monoclonal Antibody #MBS1498878
* Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510
* HA-Tag Monoclonal Antibody #MBS7135631
* E-Tag Monoclonal Antibody #MBS7135632
* TUBA1A Monoclonal Antibody #MBS7135645
* TUBB Monoclonal Antibody #MBS9018332
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
10,847 Da
NCBI Official Full Name
mRNA interferase toxin of toxin-antitoxin pair YafQ/DinJ
NCBI Official Symbol
yafQ
NCBI Official Synonym Symbols
ECK0226; JW0215
NCBI Protein Information
mRNA interferase toxin of toxin-antitoxin pair YafQ/DinJ
UniProt Protein Name
mRNA interferase toxin YafQ
Protein Family
UniProt Gene Name
yafQ

NCBI Description

YafQ is homologous to the RelE toxin, which inhibits translation as a sequence-specific mRNA endoribonuclease. [More information is available at EcoGene: EG13154]. The YafQ-DinJ toxin-antitoxin system was identified by its similarity to the RelE-RelB toxin-antitoxin system . [More information is available at EcoCyc: G6109].

Uniprot Description

Toxic component of a type II toxin-antitoxin (TA) system (PubMed:17263853). A sequence-specific mRNA endoribonuclease that inhibits translation elongation and induces bacterial stasis (PubMed:19210620). Cleavage occurs between the second and third residue of the Lys codon followed by a G or A (5'AAA(G/A)3'), is reading-frame dependent and occurs within the 5' end of most mRNAs (PubMed:19210620). Ribosome-binding confers the sequence specificity and reading frame-dependence (PubMed:19210620). When overexpressed in liquid media YafQ partially inhibits protein synthesis, with a reduction in growth rate and colony growth rate. This effect is counteracted by coexpression with cognate antitoxin DinJ (PubMed:17263853). YafQ and DinJ together bind their own promoter, and repress its expression (PubMed:24898247).

Research Articles on YAFQ

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Product Notes

The YAFQ yafq (Catalog #AAA7194470) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Escherichia coli (strain K12) YAFQ Polyclonal Antibody reacts with E Coli (strain K12) and may cross-react with other species as described in the data sheet. AAA Biotech's YAFQ can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), WB (Western Blot) (ensure identification of antigen). Researchers should empirically determine the suitability of the YAFQ yafq for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "YAFQ, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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