Rabbit anti-Human XRCC3 Polyclonal Antibody | anti-XRCC3 antibody

XRCC3 (X-ray Repair Cross-complementing Protein 3, DNA Repair Protein XRCC3, CMM6) (Biotin)

Cat. Num. AAA6398790

• Gene Names: XRCC3; CMM6
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: XRCC3; Polyclonal Antibody; XRCC3 (X-ray Repair Cross-complementing Protein 3; DNA Repair Protein XRCC3; CMM6) (Biotin); anti-XRCC3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human XRCC3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 346

• Applicable Applications for anti-XRCC3 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human XRCC3, aa1-346 (NP_005423.1).
• Immunogen Sequence: MDLDLLDLNPRIIAAIKKAKLKSVKEVLHFSGPDLKRLTNLSSPEVWHLLRTASLHLRGSSILTALQLHQQKERFPTQHQRLSLGCPVLDALLRGGLPLDGITELAGRSSAGKTQLALQLCLAVQFPRQHGGLEAGAVYICTEDAFPHKRLQQLMAQQPRLRTDVPGELLQKLRFGSQIFIEHVADVDTLLECVNKKVPVLLSRGMARLVVIDSVAAPFRCEFDSQASAPRARHLQSLGATLRELSSAFQSPVLCINQVTEAMEEQGAAHGPLGFWDERVSPALGITWANQLLVRLLADRLREEEAALGCPARTLRVLSAPHLPPSSCSYTISAEGVRGTPGTQSH
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of XRCC3 expression in transfected 293T cell line by XRCC3 polyclonal antibody. Lane 1: XRCC3 transfected lysate (37.8kD). Lane 2: Non-transfected lysate.)

Western Blot (WB)

(XRCC3 rabbit polyclonal antibody. Western Blot analysis of XRCC3 expression in K-562.)

Western Blot (WB)

(XRCC3 rabbit polyclonal antibody. Western Blot analysis of XRCC3 expression in human liver.)

Product Categories/Family for anti-XRCC3 antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 4885659
• NCBI GeneID: 7517
• NCBI Accession #: NP_005423.1
• NCBI GenBank Nucleotide #: NM_005432.2
• UniProt Accession #: O43542
• NCBI Official Full Name: DNA repair protein XRCC3
• NCBI Official Synonym Full Names: X-ray repair cross complementing 3
• NCBI Official Symbol: XRCC3
• NCBI Official Synonym Symbols: CMM6
• NCBI Protein Information: DNA repair protein XRCC3
• UniProt Protein Name: DNA repair protein XRCC3
• Protein Family: DNA repair protein
• UniProt Gene Name: XRCC3
• UniProt Entry Name: XRCC3_HUMAN

NCBI Description

This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene functionally complements Chinese hamster irs1SF, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents and is chromosomally unstable. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

XRCC3: Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C. Defects in XRCC3 are the cause of susceptibility to breast cancer (BC). BC is a common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case. Defects in XRCC3 are the cause of susceptibility to cutaneous malignant melanoma type 6 (CMM6). CMM6 is a malignant neoplasm of melanocytes, arising de novo or from a pre- existing benign nevus, which occurs most often in the skin but also may involve other sites. Belongs to the RecA family. RAD51 subfamily.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 14q32.3

Cellular Component: mitochondrion; perinuclear region of cytoplasm; cytoplasm; replication fork; nucleus

Molecular Function: DNA-dependent ATPase activity; protein binding; four-way junction DNA binding; crossover junction endodeoxyribonuclease activity; ATP binding

Biological Process: response to organic substance; DNA repair; response to DNA damage stimulus; DNA catabolic process, endonucleolytic; double-strand break repair via homologous recombination; DNA recombination

Disease: Melanoma, Cutaneous Malignant, Susceptibility To, 6; Breast Cancer