Rabbit anti-Human XBP1 Polyclonal Antibody | anti-XBP1 antibody

XBP1, ID (XBP1, TREB5, XBP2, X-box-binding protein 1, Tax-responsive element-binding protein 5)

Cat. Num. AAA6012401

• Gene Names: XBP1; XBP2; TREB5; XBP-1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: XBP1; Polyclonal Antibody; ID (XBP1; TREB5; XBP2; X-box-binding protein 1; Tax-responsive element-binding protein 5); Anti -XBP1; anti-XBP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-XBP1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: XBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-100 amino acids from the Central region of human XBP1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of XBP1 Antibody (Center) in Ramos cell line lysates (35ug/lane).XBP1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(XBP1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the XBP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(XBP1 Antibody (Center) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of XBP1 (arrow) using rabbit polyclonal XBP1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the XBP1 gene.)

Related Product Information for anti-XBP1 antibody

XBP1 encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor.

Product Categories/Family for anti-XBP1 antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 47678753
• NCBI GeneID: 7494
• UniProt Accession #: P17861; Q8WYK6; Q969P1; Q96BD7
• Molecular Weight: 28,695 Da
• NCBI Official Full Name: XBP1
• NCBI Official Synonym Full Names: X-box binding protein 1
• NCBI Official Symbol: XBP1
• NCBI Official Synonym Symbols: XBP2; TREB5; XBP-1
• NCBI Protein Information: X-box-binding protein 1; tax-responsive element-binding protein 5
• UniProt Protein Name: X-box-binding protein 1
• Protein Family: X-box-binding protein
• UniProt Gene Name: XBP1
• UniProt Synonym Gene Names: TREB5; XBP2; XBP-1
• UniProt Entry Name: XBP1_HUMAN

NCBI Description

This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5. [provided by RefSeq, Jul 2008]

Uniprot Description

XBP1: a transcription factor essential for hepatocyte growth, the differentiation of plasma cells, immunoglobulin secretion, and the unfolded protein response (UPR). XBP1 mRNA is spliced by IRE1 during the UPR to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. Only the spliced form of XBP1 can activate the UPR efficiently. Activates UPR target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I. Up-regulated by ATF6 via direct binding to the ERSE in response to endoplasmic reticulum stress. Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7). Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature. Two human isoforms are produced by alternative splicing. Isoform 1 is also known as XBP-1U. Isoform 2, also known as XBP-1S, is produced by IRE1 in response to endoplasmic reticulum stress. IRE1 cleaves a 26-bp fragment causing a frameshift of the mRNA transcript.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 22q12.1|22q12

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; endoplasmic reticulum; cytoplasm; integral to membrane; integral to endoplasmic reticulum membrane; cytosol; nucleus

Molecular Function: protein binding; protein homodimerization activity; protease binding; DNA binding; chromatin DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; estrogen receptor binding; protein kinase binding; transcription factor activity

Biological Process: ubiquitin-dependent protein catabolic process; transcription from RNA polymerase II promoter; muscle development; phosphoinositide 3-kinase cascade; apoptosis; exocrine pancreas development; positive regulation of transcription of target genes involved in unfolded protein response; regulation of protein stability; negative regulation of transcription from RNA polymerase II promoter; cellular response to glucose starvation; protein transport; serotonin secretion, neurotransmission; positive regulation of MHC class II biosynthetic process; positive regulation of transcription factor import into nucleus; angiogenesis; response to electrical stimulus; cell growth; positive regulation of TOR signaling pathway; positive regulation of autophagy; fatty acid biosynthetic process; response to drug; positive regulation of histone methylation; protein destabilization; unfolded protein response; organelle organization and biogenesis; cellular response to nutrient; positive regulation of immunoglobulin secretion; liver development; cholesterol homeostasis; positive regulation of immunoglobulin production; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; positive regulation of T cell differentiation; cellular response to insulin stimulus; endothelial cell proliferation; fatty acid homeostasis; positive regulation of fat cell differentiation; autophagy; neuron development; positive regulation of B cell differentiation; immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; sterol homeostasis; vascular endothelial growth factor receptor signaling pathway; negative regulation of apoptosis

Disease: Major Affective Disorder 7