Rabbit anti-Human WWOX Polyclonal Antibody | anti-WWOX antibody

WWOX (Phospho-Tyr33) Antibody

Cat. Num. AAA9413779

• Gene Names: WWOX; FOR; WOX1; EIEE28; FRA16D; SCAR12; HHCMA56; PRO0128; SDR41C1; D16S432E
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: WWOX; Polyclonal Antibody; WWOX (Phospho-Tyr33) Antibody; FOR; WOX1; Aberrant WW domain-containing oxidoreductase; anti-WWOX antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of WWOX only when phosphorylated at tyrosine 33.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 301

• Applicable Applications for anti-WWOX antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500~1:1000; Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of tyrosine 33 (W-V-Y(p)-Y-A) derived from Human WWOX.
• Modification: Phospho-Tyr33
• Target Name: WWOX
• Preparation and Storage: Store at -20 degree C/1 year

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells treated with PMA using WWOX (Phospho-Tyr33) Antibody.The lane on the right is treated with the antigen-specific peptide.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using WWOX (Phospho-Tyr33) antibody (left)or the same antibody preincubated with blocking peptide (right).)

Related Product Information for anti-WWOX antibody

WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 2 WW domains and a short-chain dehydrogenase/reductase domain (SRD). The highest normal expression of this gene is detected in hormonally regulated tissues such as testis, ovary, and prostate. This expression pattern and the presence of an SRD domain suggest a role for this gene in steroid metabolism. Bednarek A.K., Cancer Res. 60:2140-2145(2000).Ried K., Hum. Mol. Genet. 9:1651-1663(2000).Bednarek A.K., Cancer Res. 61:8068-8073(2001).

Product Categories/Family for anti-WWOX antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 635172870
• NCBI GeneID: 51741
• NCBI Accession #: NP_001278926.1
• NCBI GenBank Nucleotide #: NM_001291997.1
• UniProt Accession #: Q96KM3; Q5MYT5; Q96RF2; Q9BTT8; Q9NPC9; Q9NRF4; Q9NRF5; Q9NRF6; Q9NRK1; Q9NZC5; A8K323; Q9NZC7
• Molecular Weight: 23,868 Da
• NCBI Official Full Name: WW domain-containing oxidoreductase isoform 4
• NCBI Official Synonym Full Names: WW domain containing oxidoreductase
• NCBI Official Symbol: WWOX
• NCBI Official Synonym Symbols: FOR; WOX1; EIEE28; FRA16D; SCAR12; HHCMA56; PRO0128; SDR41C1; D16S432E
• NCBI Protein Information: WW domain-containing oxidoreductase
• UniProt Protein Name: WW domain-containing oxidoreductase
• Protein Family: WW domain-containing oxidoreductase
• UniProt Gene Name: WWOX
• UniProt Synonym Gene Names: FOR; SDR41C1; WOX1
• UniProt Entry Name: WWOX_HUMAN

NCBI Description

This gene encodes a member of the short-chain dehydrogenases/reductases (SDR) protein family. This gene spans the FRA16D common chromosomal fragile site and appears to function as a tumor suppressor gene. Expression of the encoded protein is able to induce apoptosis, while defects in this gene are associated with multiple types of cancer. Disruption of this gene is also associated with autosomal recessive spinocerebellar ataxia 12. Disruption of a similar gene in mouse results in impaired steroidogenesis, additionally suggesting a metabolic function for the protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

WWOX: an enzyme which contains 2 WW domains and a short-chain dehydrogenase/reductase domain (SRD). Expressed at high levels in hormonally regulated tissues such as testis, ovary, and prostate. Its SRD domain suggest a role for this gene in steroid metabolism. Shown to be an essential mediator of tumor necrosis factor-alpha-induced apoptosis in the mouse. May play a similar role in the human. May function as a suppressor of tumor growth. Alternative splicing of this gene generates 7 isoforms.

Protein type: Apoptosis; Tumor suppressor; EC 1.1.1.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 16q23

Cellular Component: Golgi apparatus; microvillus; mitochondrion; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function: protein dimerization activity; protein binding; enzyme binding; oxidoreductase activity; cofactor binding; coenzyme binding

Biological Process: osteoblast differentiation; steroid metabolic process; negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway; skeletal morphogenesis; positive regulation of transcription from RNA polymerase II promoter

Disease: Epileptic Encephalopathy, Early Infantile, 28