Rabbit anti-Mouse WWOX Polyclonal Antibody | anti-WWOX antibody

WWOX, CT (WWOX, FOR, WOX1, WW domain-containing oxidoreductase, Fragile site FRA16D oxidoreductase) (APC)

Cat. Num. AAA6363853

• Gene Names: WWOX; FOR; WOX1; FRA16D; HHCMA56; PRO0128; SDR41C1; D16S432E
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: WWOX; Polyclonal Antibody; CT (WWOX; FOR; WOX1; WW domain-containing oxidoreductase; Fragile site FRA16D oxidoreductase) (APC); Fragile site FRA16D oxidoreductase; anti-WWOX antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-WWOX antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: WWOX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 368-396 amino acids from the C-terminal region of human WWOX.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(WWOX Antibody (C-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the WWOX antibody detected the WWOX protein (arrow).)

Related Product Information for anti-WWOX antibody

WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 2 WW domains and a short-chain dehydrogenase/reductase domain (SRD). The highest normal expression of this gene is detected in hormonally regulated tissues such as testis, ovary, and prostate. This expression pattern and the presence of an SRD domain suggest a role for this gene in steroid metabolism. The encoded protein is more than 90% identical to the mouse protein, which is an essential mediator of tumor necrosis factor-alpha-induced apoptosis, suggesting a similar, important role in apoptosis for the human protein. In addition, there is evidence that this gene behaves as a suppressor of tumor growth. Alternative splicing of this gene generates transcript variants that encode different isoforms.

Product Categories/Family for anti-WWOX antibody

Antibodies; Enzymes; Reductase

References

Maeda, N., et al. Virchows Arch. 457(4):423-432(2010). Baykara, O., et al. Tumour Biol. 31(4):315-320(2010). Kurek, K.C., et al. Cancer Res. 70(13):5577-5586(2010). Lin, J., et al. Zhonghua Gan Zang Bing Za Zhi 18(5):357-360(2010). Saez, M.E., et al. BMC Med. Genet. 11, 148 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 7706523
• NCBI GeneID: 51741
• NCBI Accession #: NP_057457.1; NP_570607.1
• NCBI GenBank Nucleotide #: NM_016373.2
• UniProt Accession #: Q9NZC7; Q5MYT5; Q96KM3; Q96RF2; Q9BTT8; Q9NPC9; Q9NRF4; Q9NRF5; Q9NRF6; Q9NRK1; Q9NZC5; A8K323
• Molecular Weight: 46,677 Da
• NCBI Official Full Name: WW domain-containing oxidoreductase isoform 1
• NCBI Official Synonym Full Names: WW domain containing oxidoreductase
• NCBI Official Symbol: WWOX
• NCBI Official Synonym Symbols: FOR; WOX1; FRA16D; HHCMA56; PRO0128; SDR41C1; D16S432E
• NCBI Protein Information: WW domain-containing oxidoreductase; WW domain-containing protein WWOX; fragile site FRA16D oxidoreductase; short chain dehydrogenase/reductase family 41C, member 1
• UniProt Protein Name: WW domain-containing oxidoreductase
• Protein Family: WW domain-containing oxidoreductase
• UniProt Gene Name: WWOX
• UniProt Synonym Gene Names: FOR; WOX1
• UniProt Entry Name: WWOX_HUMAN

NCBI Description

WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 2 WW domains and a short-chain dehydrogenase/reductase domain (SRD). The highest normal expression of this gene is detected in hormonally regulated tissues such as testis, ovary, and prostate. This expression pattern and the presence of an SRD domain suggest a role for this gene in steroid metabolism. The encoded protein is more than 90% identical to the mouse protein, which is an essential mediator of tumor necrosis factor-alpha-induced apoptosis, suggesting a similar, important role in apoptosis for the human protein. In addition, there is evidence that this gene behaves as a suppressor of tumor growth. Alternative splicing of this gene generates transcript variants that encode different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

WWOX: an enzyme which contains 2 WW domains and a short-chain dehydrogenase/reductase domain (SRD). Expressed at high levels in hormonally regulated tissues such as testis, ovary, and prostate. Its SRD domain suggest a role for this gene in steroid metabolism. Shown to be an essential mediator of tumor necrosis factor-alpha-induced apoptosis in the mouse. May play a similar role in the human. May function as a suppressor of tumor growth. Alternative splicing of this gene generates 7 isoforms.

Protein type: Tumor suppressor; Oxidoreductase; EC 1.1.1.-; Apoptosis

Chromosomal Location of Human Ortholog: 16q23

Cellular Component: Golgi apparatus; microvillus; mitochondrion; cytoplasm; plasma membrane; nucleus; cytosol

Molecular Function: protein dimerization activity; protein binding; enzyme binding; oxidoreductase activity; coenzyme binding; cofactor binding

Biological Process: osteoblast differentiation; steroid metabolic process; Wnt receptor signaling pathway; negative regulation of Wnt receptor signaling pathway; skeletal morphogenesis; positive regulation of transcription from RNA polymerase II promoter

Disease: Epileptic Encephalopathy, Early Infantile, 28