Rabbit WWOX Polyclonal Antibody | anti-WWOX antibody

WWOX antibody

Cat. Num. AAA536450

• Gene Names: WWOX; FOR; WOX1; EIEE28; FRA16D; SCAR12; HHCMA56; PRO0128; SDR41C1; D16S432E
• Applications: Western Blot
• Synonyms: WWOX; Polyclonal Antibody; WWOX antibody; Polyclonal WWOX; Anti-WWOX; WOX1; FOR; WW domain containing oxidoreductase; PRO0128; SDR41C1; D16S432E; HHCMA56; FRA16D; anti-WWOX antibody

• Host: Rabbit
• Clonality: Polyclonal
• Form/Format: Lyophilized powder. Add 50 ul of distilled water. Final antibody concentration is 1 mg/ml in PBS buffer.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 414

• Applicable Applications for anti-WWOX antibody: Western Blot (WB)
• Application Notes: Optimal conditions to be determined by the end user
• Biological Significance: WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 2 WW domains and a short-chain dehydrogenase/reductase domain (SRD). The highest normal expression of this gene is detected in hormonally regulated tissues such as testis, ovary, and prostate. This expression pattern and the presence of an SRD domain suggest a role for this gene in steroid metabolism. The encoded protein is more than 90% identical to the mouse protein, which is an essential mediator of tumor necrosis factor-alpha-induced apoptosis, suggesting a similar, important role in apoptosis for the human protein. In addition, there is evidence that this gene behaves as a suppressor of tumor growth. Alternative splicing of this gene generates transcript variants that encode different isoforms.
• Cross-Reactivity: Human
• Immunogen: WWOX antibody was raised in rabbit using the C terminal of WWOX as the immunogen
• Preparation and Storage: Store at 4 degree C, following reconstitution, aliquot and store at -20 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot showing WWOX antibody used at a concentration of 1 ug/ml against COLO205 Cell Lysate)

Related Product Information for anti-WWOX antibody

Affinity purified rabbit polyclonal WWOX antibody

Product Categories/Family for anti-WWOX antibody

Cell Cycle & Cell Death; Purified Polyclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 7706523
• NCBI GeneID: 51741
• NCBI Accession #: NP_057457.1
• NCBI GenBank Nucleotide #: NM_016373.2
• UniProt Accession #: Q9NZC7; Q5MYT5; Q96KM3; Q96RF2; Q9BTT8; Q9NPC9; Q9NRF4; Q9NRF5; Q9NRF6; Q9NRK1; Q9NZC5; A8K323
• Molecular Weight: 23,868 Da
• NCBI Official Full Name: WW domain-containing oxidoreductase isoform 1
• NCBI Official Synonym Full Names: WW domain containing oxidoreductase
• NCBI Official Symbol: WWOX
• NCBI Official Synonym Symbols: FOR; WOX1; EIEE28; FRA16D; SCAR12; HHCMA56; PRO0128; SDR41C1; D16S432E
• NCBI Protein Information: WW domain-containing oxidoreductase
• UniProt Protein Name: WW domain-containing oxidoreductase
• Protein Family: WW domain-containing oxidoreductase
• UniProt Gene Name: WWOX
• UniProt Synonym Gene Names: FOR; SDR41C1; WOX1
• UniProt Entry Name: WWOX_HUMAN

NCBI Description

This gene encodes a member of the short-chain dehydrogenases/reductases (SDR) protein family. This gene spans the FRA16D common chromosomal fragile site and appears to function as a tumor suppressor gene. Expression of the encoded protein is able to induce apoptosis, while defects in this gene are associated with multiple types of cancer. Disruption of this gene is also associated with autosomal recessive spinocerebellar ataxia 12. Disruption of a similar gene in mouse results in impaired steroidogenesis, additionally suggesting a metabolic function for the protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

WWOX: an enzyme which contains 2 WW domains and a short-chain dehydrogenase/reductase domain (SRD). Expressed at high levels in hormonally regulated tissues such as testis, ovary, and prostate. Its SRD domain suggest a role for this gene in steroid metabolism. Shown to be an essential mediator of tumor necrosis factor-alpha-induced apoptosis in the mouse. May play a similar role in the human. May function as a suppressor of tumor growth. Alternative splicing of this gene generates 7 isoforms.

Protein type: Tumor suppressor; EC 1.1.1.-; Apoptosis; Oxidoreductase

Chromosomal Location of Human Ortholog: 16q23

Cellular Component: Golgi apparatus; microvillus; mitochondrion; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function: protein dimerization activity; protein binding; enzyme binding; oxidoreductase activity; coenzyme binding; cofactor binding

Biological Process: steroid metabolic process; osteoblast differentiation; Wnt receptor signaling pathway; negative regulation of Wnt receptor signaling pathway; skeletal morphogenesis; positive regulation of transcription from RNA polymerase II promoter

Disease: Epileptic Encephalopathy, Early Infantile, 28