Rabbit anti-Human, Mouse WNT5A Polyclonal Antibody | anti-WNT5A antibody

WNT5A Antibody

Cat. Num. AAA7049380

• Gene Names: WNT5A; hWNT5A
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: WNT5A; Polyclonal Antibody; WNT5A Antibody; Protein Wnt-5a; anti-WNT5A antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 365

• Applicable Applications for anti-WNT5A antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1: 500-1: 5000; IHC: 1: 20-1: 200; IF: 1: 50-1: 200
• Immunogen: Recombinant Human Protein Wnt-5a protein (62-380AA)
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Conjugation: Non-conjugated
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-23698 / sc-30224
• Immunogen Species: Homo Sapiens (Human)
• Research Area: Neuroscience
• Target Names: WNT5A
• Preparation and Storage: Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Mouse heart tissueAll lanes: WNT5A antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 43,41 KDaObserved band size: 43 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung cancer using MBS7049380 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human tonsil tissue using MBS7049380 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of PC3 cells using MBS7049380 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-WNT5A antibody

WNT5A polyclonal antibody MBS7049380 was produced in the rabbits immunized by using the Recombinant Human Protein Wnt-5a protein (62- 380AA) as the immunogen. The target protein WNT5A is ligand for members of the frizzled family of seven transmembrane receptors. It can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, it activates beta-catenin signaling. In the presence of ROR2, it inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway. This Rabbit anti-Homo sapiens (Human) WNT5A Polyclonal antibody was tested in the ELISA, WB, IHC and IF applications. The non-conjugated IgG got purified by protein G and reached up to 95% in purity. It reacts with the WNT5A proteins of human or mouse-origin and may be used to detect the endogenous levels of WNT5A protein.

NCBI and Uniprot Product Information

• NCBI GI #: 371502087
• NCBI GeneID: 7474
• NCBI Accession #: NP_001243034.1
• NCBI GenBank Nucleotide #: NM_001256105.1
• UniProt Accession #: P41221; Q6P278; A8K4A4
• NCBI Official Full Name: protein Wnt-5a isoform 2
• NCBI Official Synonym Full Names: Wnt family member 5A
• NCBI Official Symbol: WNT5A
• NCBI Official Synonym Symbols: hWNT5A
• NCBI Protein Information: protein Wnt-5a
• UniProt Protein Name: Protein Wnt-5a
• Protein Family: Protein
• UniProt Gene Name: WNT5A
• UniProt Entry Name: WNT5A_HUMAN

NCBI Description

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

WNT5A: Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes. Interacts with PORCN. Interacts with WLS. Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells. Belongs to the Wnt family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3p21-p14

Cellular Component: endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi lumen; plasma membrane

Molecular Function: frizzled binding; protein binding; receptor agonist activity; receptor tyrosine kinase-like orphan receptor binding; transcription factor activity

Biological Process: activation of JNK activity; activation of MAPK activity; activation of NF-kappaB transcription factor; activation of protein kinase B; axon guidance; cell fate commitment; embryonic skeletal development; epithelial to mesenchymal transition; genitalia development; keratinocyte differentiation; lens development in camera-type eye; male gonad development; negative regulation of apoptosis; negative regulation of fat cell differentiation; negative regulation of transcription, DNA-dependent; neuron differentiation; olfactory bulb interneuron development; palate development; positive regulation of angiogenesis; positive regulation of cGMP metabolic process; positive regulation of chemokine biosynthetic process; positive regulation of cytokine secretion during immune response; positive regulation of endothelial cell proliferation; positive regulation of fibroblast proliferation; positive regulation of inflammatory response; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-6 production; positive regulation of macrophage activation; positive regulation of ossification; positive regulation of protein catabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; response to organic substance; Wnt receptor signaling pathway; Wnt receptor signaling pathway, calcium modulating pathway; Wnt receptor signaling pathway, planar cell polarity pathway; wound healing

Disease: Robinow Syndrome, Autosomal Dominant