Rabbit anti-Human, Mouse Wnt10B Polyclonal Antibody | anti-Wnt10B antibody

Wnt10B, ID (Protein Wnt-10b, Wnt-12, Wnt12) (PE)

Cat. Num. AAA6506245

• Gene Names: WNT10B; SHFM6; WNT-12
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Wnt10B; Polyclonal Antibody; ID (Protein Wnt-10b; Wnt-12; Wnt12) (PE); anti-Wnt10B antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human WNT10B. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-Wnt10B antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human WNT10B (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of WNT10B Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). WNT10B (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of WNT10B Antibody (Center) in mouse liver tissue lysates (35ug/lane). WNT10B (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(WNT10B Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WNT10B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of MDA-231 cells using WNT10B Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-Wnt10B antibody

Antibodies; Wnt Proteins

References

1. Zmuda, J.M., J. Bone Miner. Res. 24(3), 437-447 (2009). 2. Pederson, L., Proc. Natl. Acad. Sci. USA 105(52), 20764-20769 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 16936522
• NCBI GeneID: 7480
• NCBI Accession #: NP_003385
• NCBI GenBank Nucleotide #: NM_003394.3
• UniProt Accession #: O00744; O00747; Q4VAJ4; Q4VAJ5; Q8WZ97; B2R7A5
• Molecular Weight: Predicted: 43 kDa; ; Observed: 47 kDa
• NCBI Official Full Name: protein Wnt-10b
• NCBI Official Synonym Full Names: wingless-type MMTV integration site family, member 10B
• NCBI Official Symbol: WNT10B
• NCBI Official Synonym Symbols: SHFM6; WNT-12
• NCBI Protein Information: protein Wnt-10b; WNT-10B protein
• UniProt Protein Name: Protein Wnt-10b
• UniProt Gene Name: WNT10B
• UniProt Synonym Gene Names: WNT12
• UniProt Entry Name: WN10B_HUMAN

NCBI Description

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level. This gene is clustered with another family member, WNT1, in the chromosome 12q13 region. [provided by RefSeq, Jul 2008]

Uniprot Description

WNT10B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Defects in WNT10B are the cause of split-hand/foot malformation type 6 (SHFM6). SHFM is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. SHFM6 is a autosomal recessive disorder. Belongs to the Wnt family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: frizzled binding; receptor agonist activity

Biological Process: positive regulation of apoptosis; skeletal muscle fiber development; Wnt receptor signaling pathway through beta-catenin; negative regulation of transcription from RNA polymerase II promoter; neuron differentiation; positive regulation of cell proliferation; positive regulation of epithelial cell differentiation; chondrocyte differentiation; positive regulation of transcriptional preinitiation complex assembly; G2/M transition of mitotic cell cycle; cell cycle arrest; negative regulation of epithelial cell proliferation; smoothened signaling pathway; cell fate commitment; Wnt receptor signaling pathway; protein stabilization; sensory perception of taste; regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of bone mineralization; negative regulation of fat cell differentiation; positive regulation of osteoblast differentiation; positive regulation of transcription factor activity; regulation of skeletal muscle development; lipid metabolic process; positive regulation of anagen; myoblast cell differentiation involved in skeletal muscle regeneration

Disease: Split-hand/foot Malformation 6