Western Blot (WB)
(Western blot analysis of extracts of Raji cells, using Phospho-WIPI2 (Ser413) Antibody.)
Rabbit WIPI2 Polyclonal Antibody | anti-WIPI2 antibody
Phospho-WIPI2 (Ser413) Antibody
Gene Names
WIPI2; Atg21; ATG18B; CGI-50; WIPI-2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Synonyms
WIPI2; Polyclonal Antibody; Phospho-WIPI2 (Ser413) Antibody; ATG18B; Atg21; CGI 50; DKFZp434J154; DKFZp686P02188; FLJ12979; FLJ14217; FLJ42984; WD repeat domain phosphoinositide-interacting protein 2; WD repeat domain; phosphoinositide interacting 2; WD40 repeat protein interacting with phosphoinositides 2; WIPI 2; WIPI-2; WIPI2_HUMAN; WIPI49 like protein 2; WIPI49-like protein 2; anti-WIPI2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-WIPI2 (Ser413) antibody detects endogenous levels of WIPI2.
Purity/Purification
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
443
Applicable Applications for anti-WIPI2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human WIPI2 around the phosphorylation site of Ser413.
Subcellular Location
Preautophagosomal Structure Membrane. Enriched at preautophagosomal structure membranes in response to ptdIns3P.
Tissue Specificity
Ubiquitously expressed (at protein level). Highly expressed in heart, skeletal muscle and pancreas. Expression is down-regulated in pancreatic and in kidney tumors.
Predicted Cross Reactivity
Pig, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (90%), Sheep (82%), Rabbit (91%), Dog (92%), Chicken (92%)
Conjugation
Unconjugated
Epitope
Phospho Ser413
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts of Raji cells, using Phospho-WIPI2 (Ser413) Antibody.)
Western Blot (WB)
(Western blot analysis of extracts of Raji cells, using Phospho-WIPI2 (Ser413) Antibody.)
Immunohistochemistry (IHC)
(MBS9600762 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9600762 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-WIPI2 antibody
Function: Early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to phosphatidylinositol 3-phosphate (PtdIns3P). May bind PtdIns3P.
Subunit Structure: Interacts with TECPR1, ATG16L1 and ATG5.
Similarity: The FRRG motif is required for recruitment to PtdIns3P. Belongs to the WD repeat SVP1 family.
Subunit Structure: Interacts with TECPR1, ATG16L1 and ATG5.
Similarity: The FRRG motif is required for recruitment to PtdIns3P. Belongs to the WD repeat SVP1 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 49 kDa
Predicted: 50 kDa
Predicted: 50 kDa
NCBI Official Full Name
WD repeat domain phosphoinositide-interacting protein 2 isoform c
NCBI Official Synonym Full Names
WD repeat domain, phosphoinositide interacting 2
NCBI Official Symbol
WIPI2
NCBI Official Synonym Symbols
Atg21; ATG18B; CGI-50; WIPI-2
NCBI Protein Information
WD repeat domain phosphoinositide-interacting protein 2
UniProt Protein Name
WD repeat domain phosphoinositide-interacting protein 2
Protein Family
UniProt Gene Name
WIPI2
UniProt Synonym Gene Names
WIPI-2
NCBI Description
WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM, Mar 2008]
Uniprot Description
Early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to phosphatidylinositol 3-phosphate (PtdIns3P). May bind PtdIns3P.
Research Articles on WIPI2
Similar Products
Product Notes
The WIPI2 wipi2 (Catalog #AAA9600762) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-WIPI2 (Ser413) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's WIPI2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the WIPI2 wipi2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WIPI2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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