Rabbit anti-Human WIPI2 Polyclonal Antibody | anti-WIPI2 antibody

WIPI2 Antibody

Cat. Num. AAA809778

• Gene Names: WIPI2; Atg21; ATG18B; CGI-50; WIPI-2
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Peptide Affinity Purified
• Synonyms: WIPI2; Polyclonal Antibody; WIPI2 Antibody; Rabbit Anti-Human WIPI2 Polyclonal; WD repeat domain phosphoinositide-interacting protein 2 Antibody; WIPI-2 Antibody; WIPI49-like protein 2 Antibody; CGI-50 Antibody; ATG21 Antibody; WIPI 2 Antibody; ATG18B Antibody; anti-WIPI2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Detects ~55 kDa.
• Purity/Purification: Peptide Affinity Purified
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 443

• Applicable Applications for anti-WIPI2 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:1000; ICC/IF: 1:100
• Target: WIPI2
• Immunogen: Synthetic peptide from the C-terminal of Human WIPI2
• Immunogen Species: Human
• Cellular Localization: Preautophagosomal Structure Membrane; Peripheral Membrane Protein; Cytoplasmic Side
• Conjugate: Unconjugated
• Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide
• Tissue Specificity: Ubiquitously expressed (at protein level). Highly expressed in heart, skeletal muscle and pancreas. Expression is down-regulated in pancreatic and in kidney tumors.
• Preparation and Storage: Store at -20 degree C.

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-WIPI2 Polyclonal Antibody . Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-WIPI2 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) WIPI2 Antibody (D) Composite.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-WIPI2 Polyclonal Antibody . Tissue: Heart. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-WIPI2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)

Western Blot (WB)

(Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of ~49.4 kDa WIPI2 protein using Rabbit Anti-WIPI2 Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Lane 3: 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-WIPI2 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~49.4 kDa.)

Product Categories/Family for anti-WIPI2 antibody

Antibodies; Cancer; Autophagy

NCBI and Uniprot Product Information

• NCBI GI #: 75677329
• NCBI GeneID: 26100
• NCBI Accession #: NP_001028690.1
• NCBI GenBank Nucleotide #: NM_001033518.1
• UniProt Accession #: Q9Y4P8; Q5MNZ8; Q6FI96; Q75L50; Q96IE4; Q9Y364; B3KNC2
• Molecular Weight: 48,249 Da
• NCBI Official Full Name: WD repeat domain phosphoinositide-interacting protein 2 isoform c
• NCBI Official Synonym Full Names: WD repeat domain, phosphoinositide interacting 2
• NCBI Official Symbol: WIPI2
• NCBI Official Synonym Symbols: Atg21; ATG18B; CGI-50; WIPI-2
• NCBI Protein Information: WD repeat domain phosphoinositide-interacting protein 2
• UniProt Protein Name: WD repeat domain phosphoinositide-interacting protein 2
• Protein Family: WD repeat domain phosphoinositide-interacting protein
• UniProt Gene Name: WIPI2
• UniProt Synonym Gene Names: WIPI-2

NCBI Description

WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM, Mar 2008]

Uniprot Description

Early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to phosphatidylinositol 3-phosphate (PtdIns3P). May bind PtdIns3P.