Rabbit WDR77 Polyclonal Antibody | anti-WDR77 antibody

WDR77 Antibody

Cat. Num. AAA9629694

• Gene Names: WDR77; p44; MEP50; MEP-50; HKMT1069; Nbla10071; p44/Mep50
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: WDR77; Polyclonal Antibody; WDR77 Antibody; 2610312E17Rik; Androgen receptor cofactor p44; C79984; HKMT1069; MEP 50; MEP-50; MEP50; MEP50_HUMAN; Methylosome protein 50; MGC2722; Nbla10071; p44; p44/Mep50; RGD1310479; RP11 552M11.3; WD repeat containing protein 77; WD repeat domain 77; WD repeat-containing protein 77; anti-WDR77 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: WDR77 Antibody detects endogenous levels of total WDR77.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-WDR77 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Subunit Structure: Component of the methylosome complex composed of PRMT5, WDR77 and CLNS1A. Found in a complex composed of PRMT5, WDR77 and RIOK1. RIOK1 and CLNS1A bound directly to PRMT5 at the same binding site, in a mutually exclusive manner, which allows the recruitment of distinct methylation substrates, such as nucleolin/NCL and Sm proteins, respectively. Found in a complex with the component of the methylosome, PRMT5, CLNS1A, WDR77, PRMT1 and ERH. Directly interacts with PRMT5, as well as with several Sm proteins, including SNRPB and SNRPD2 and, more weakly, SNRPD3 and SNRPE. Forms a compact hetero-octamer with PRMT5, decorating the outer surface of a PRMT5 tetramer. Interacts with SUZ12 and histone H2A/HIST2H2AC, but not with histones H2B, H3 nor H4. Interacts with CTDP1 and LSM11. Interacts with APEX1, AR and NKX3-1. Interacts with CHTOP.
• Tissue Specificity: Highly expressed in heart, skeletal muscle, spleen, testis, uterus, prostate and thymus. In testis, expressed in germ cells and Leydig cells, but not in peritubular myocytes, nor in Sertoli cells. Expressed in prostate cancers, in seminomas and in Leydig cell tumors.
• Function: Non-catalytic component of the methylosome complex, composed of PRMT5, WDR77 and CLNS1A, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The methylosome complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.
• Note: Nuclear in Leydig cells and cytoplasmic in germ cells during fetal testicular development. In adult testis, predominantly nuclear. Subcellular location varies from nuclear to cytoplasmic in various tumors (PubMed:17437848).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-WDR77 antibody

Nucleus; Cytoplasm; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 13129110
• NCBI GeneID: 79084
• NCBI Accession #: NP_077007.1
• NCBI GenBank Nucleotide #: NM_024102.2
• UniProt Accession #: Q9BQA1
• NCBI Official Full Name: methylosome protein 50 isoform 2
• NCBI Official Synonym Full Names: WD repeat domain 77
• NCBI Official Symbol: WDR77
• NCBI Official Synonym Symbols: p44; MEP50; MEP-50; HKMT1069; Nbla10071; p44/Mep50
• NCBI Protein Information: methylosome protein 50
• UniProt Protein Name: Methylosome protein 50
• Protein Family: Methylosome protein
• UniProt Gene Name: WDR77
• UniProt Synonym Gene Names: MEP50; WD45; MEP-50
• UniProt Entry Name: MEP50_HUMAN

NCBI Description

The protein encoded by this gene is an androgen receptor coactivator that forms a complex with protein arginine methyltransferase 5, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. The encoded protein may be involved in the early stages of prostate cancer, with most of the protein being nuclear-localized in benign cells but cytoplasmic in cancer cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

Uniprot Description

WDR77: a WD40 repeat protein. Component of the methylosome, a 20S complex containing at least ICLN, SKB1 and WDR77. Interacts with SKB1 and with SM proteins. The methylosome may regulate an early step in the assembly of U snRNPs, possibly the transfer of Sm proteins to the SMN-complex. WD40 repeats are found in a number of eukaryotic proteins that coordinate multi-protein complex assemblies. WD40 proteins are implicated in many functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly.

Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; ligand-dependent nuclear receptor transcription coactivator activity

Biological Process: regulation of transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; spliceosomal snRNP biogenesis; positive regulation of cell proliferation; gene expression