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Western Blot (WB) (WDR73 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane). This demonstrates the WDR73 antibody detected the WDR73 protein (arrow).)

Rabbit anti-Human WDR73 Polyclonal Antibody | anti-WDR73 antibody

WDR73, NT (WDR73, WD repeat-containing protein 73) (FITC)

Gene Names
WDR73; HSPC264
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
WDR73; Polyclonal Antibody; NT (WDR73; WD repeat-containing protein 73) (FITC); WD repeat-containing protein 73; anti-WDR73 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-WDR73 antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
WDR73 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-82 amino acids from the N-terminal region of human WDR73.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(WDR73 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane). This demonstrates the WDR73 antibody detected the WDR73 protein (arrow).)

Western Blot (WB) (WDR73 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane). This demonstrates the WDR73 antibody detected the WDR73 protein (arrow).)

Immunohistochemistry (IHC)

(WDR73 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WDR73 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (WDR73 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WDR73 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(WDR73 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (WDR73 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-WDR73 antibody
References
Suzuki, Y., et al. Gene 200 (1-2), 149-156 (1997).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
41,685 Da
NCBI Official Full Name
WDR73 protein, partial
NCBI Official Synonym Full Names
WD repeat domain 73
NCBI Official Symbol
WDR73
NCBI Official Synonym Symbols
HSPC264
NCBI Protein Information
WD repeat-containing protein 73; FLJ00296 protein
UniProt Protein Name
WD repeat-containing protein 73
UniProt Gene Name
WDR73
UniProt Entry Name
WDR73_HUMAN

NCBI Description

The protein encoded by this gene is thought to contain multiple WD40 repeats. WD40 repeats are motifs that contain 40-60 amino acids, and usually end with Trp-Asp (WD). This protein is found in the cytoplasm during interphase, but accumulates at the spindle poles and astral microtubules during mitosis. Reduced expression of this gene results in abnormalities in the size and morphology of the nucleus. Mutations in this gene have been associated with Galloway-Mowat syndrome PMID: 25466283), which is a rare autosomal recessive disorder that affects both the central nervous system and kidneys. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]

Uniprot Description

WDR73: Belongs to the WD repeat WDR73 family.

Chromosomal Location of Human Ortholog: 15q25.2

Disease: Galloway-mowat Syndrome

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Product Notes

The WDR73 wdr73 (Catalog #AAA6363404) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The WDR73, NT (WDR73, WD repeat-containing protein 73) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's WDR73 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the WDR73 wdr73 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WDR73, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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