Rabbit anti-Human WDR61 Polyclonal Antibody | anti-WDR61 antibody

WDR61, ID (WDR61, WD repeat-containing protein 61, Meiotic recombination REC14 protein homolog, SKI8 homolog) (PE)

Cat. Num. AAA6363378

• Gene Names: WDR61; SKI8; REC14
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: WDR61; Polyclonal Antibody; ID (WDR61; WD repeat-containing protein 61; Meiotic recombination REC14 protein homolog; SKI8 homolog) (PE); SKI8 homolog; anti-WDR61 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-WDR61 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: WDR61 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-149 amino acids from the Central region of human WDR61.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(WDR61 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the WDR61 antibody detected the WDR61 protein (arrow).)

Related Product Information for anti-WDR61 antibody

WDR61 is a subunit of the human PAF and SKI complexes, which function in transcriptional regulation and are involved in events downstream of RNA synthesis, such as RNA surveillance (Zhu et al., 2005 [PubMed 16024656]).

Product Categories/Family for anti-WDR61 antibody

Antibodies; WD Repeat Proteins

References

Lamesch, P., et al. Genomics 89(3):307-315(2007). Zhu, B., et al. Genes Dev. 19(14):1668-1673(2005). Barrios-Rodiles, M., et al. Science 307(5715):1621-1625(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 13376840
• NCBI GeneID: 80349
• NCBI Accession #: NP_079510.1
• NCBI GenBank Nucleotide #: NM_025234.2
• UniProt Accession #: Q9GZS3; Q6IA22; Q7Z4X4; D3DW84
• Molecular Weight: 33,581 Da
• NCBI Official Full Name: WD repeat-containing protein 61 isoform a
• NCBI Official Synonym Full Names: WD repeat domain 61
• NCBI Official Symbol: WDR61
• NCBI Official Synonym Symbols: SKI8; REC14
• NCBI Protein Information: WD repeat-containing protein 61
• UniProt Protein Name: WD repeat-containing protein 61
• Protein Family: WD repeat-containing protein
• UniProt Gene Name: WDR61
• UniProt Synonym Gene Names: Ski8
• UniProt Entry Name: WDR61_HUMAN

NCBI Description

WDR61 is a subunit of the human PAF and SKI complexes, which function in transcriptional regulation and are involved in events downstream of RNA synthesis, such as RNA surveillance (Zhu et al., 2005 [PubMed 16024656]).[supplied by OMIM, Mar 2008]

Uniprot Description

WDR61: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of MLL1; it promotes leukemogenesis though association with MLL-rearranged oncoproteins, such as MLL-MLLT3/AF9 and MLL-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Required for mono- and trimethylation on histone H3 'Lys-4' (H3K4me3), dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1C.

Chromosomal Location of Human Ortholog: 15q25.1

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: Wnt receptor signaling pathway; negative regulation of myeloid cell differentiation; transcription, DNA-dependent; positive regulation of RNA elongation from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; positive regulation of histone H3-K4 methylation