Rabbit anti-Human WDR48 Polyclonal Antibody | anti-WDR48 antibody

WDR48, CT (WDR48, KIAA1449, UAF1, WD repeat-containing protein 48, USP1-associated factor 1, WD repeat endosomal protein, p80) (PE)

Cat. Num. AAA6363312

• Gene Names: WDR48; P80; UAF1; SPG60
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: WDR48; Polyclonal Antibody; CT (WDR48; KIAA1449; UAF1; WD repeat-containing protein 48; USP1-associated factor 1; WD repeat endosomal protein; p80) (PE); p80; anti-WDR48 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-WDR48 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: WDR48 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 610-638 amino acids from the C-terminal region of human WDR48.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(WDR48 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the WDR48 antibody detected the WDR48 protein (arrow).)

Immunohistochemistry (IHC)

(WDR48 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WDR48 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(WDR48 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-WDR48 antibody

Regulator of deubiquitinating complexes. Acts as a strong activator of USP1 by enhancing the USP1-mediated deubiquitination of FANCD2; USP1 being almost inactive by itself. Also activates deubiquitinating activity of complexes containing USP12 and USP46, respectively. Activates deubiquitination by increasing the catalytic turnover without increasing the affinity of deubiquitinating enzymes for the substrate. In case of infection by Herpes virus saimiri, may play a role in vesicular transport or membrane fusion events necessary for transport to lysosomes. Induces lysosomal vesicle formation via interaction with Herpes virus saimiri tyrosine kinase-interacting protein (TIP). Subsequently, TIP recruits tyrosine-protein kinase LCK, resulting in down-regulation of T-cell antigen receptor TCR. May play a role in generation of enlarged endosomal vesicles via interaction with TIP. In case of infection by papillomavirus HPV11, promotes the maintenance of the viral genome via its interaction with HPV11 helicase E1.

Product Categories/Family for anti-WDR48 antibody

Antibodies; WD Repeat Proteins

References

Kee, Y., et al. J. Biol. Chem. 285(15):11252-11257(2010). Cohn, M.A., et al. J. Biol. Chem. 284(8):5343-5351(2009). Cote-Martin, A., et al. J. Virol. 82(3):1271-1283(2008). Cohn, M.A., et al. Mol. Cell 28(5):786-797(2007). Park, J., et al. J. Virol. 77(16):9041-9051(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 18874090
• NCBI GeneID: 57599
• NCBI Accession #: NP_065890.1
• NCBI GenBank Nucleotide #: NM_020839.3
• UniProt Accession #: Q8TAF3; Q63HJ2; Q658Y1; Q8N3Z1; Q9NSK8; Q9P279; B4DM86; B4DQI2; B4DY84
• Molecular Weight: 45,552 Da
• NCBI Official Full Name: WD repeat-containing protein 48 isoform 1
• NCBI Official Synonym Full Names: WD repeat domain 48
• NCBI Official Symbol: WDR48
• NCBI Official Synonym Symbols: P80; UAF1; SPG60
• NCBI Protein Information: WD repeat-containing protein 48
• UniProt Protein Name: WD repeat-containing protein 48
• Protein Family: WD repeat-containing protein
• UniProt Gene Name: WDR48
• UniProt Synonym Gene Names: KIAA1449; UAF1
• UniProt Entry Name: WDR48_HUMAN

Uniprot Description

WDR48: an ubiquitous lysosomal WD40 repeat protein. May play a role in vesicular transport or membrane fusion events necessary for transport to lysosomes. Induces lysosomal vesicle formation via interaction with Herpesvirus saimiri tyrosine kinase-interacting protein (TIP). Subsequently, TIP recruits tyrosine-protein kinase LCK, resulting in down-regulation of T-cell antigen receptor TCR. May play a role in generation of enlarged endosomal vesicles via interaction with TIP.

Protein type: Ubiquitin conjugating system; Vesicle

Chromosomal Location of Human Ortholog: 3p21.33

Cellular Component: intracellular membrane-bound organelle; lysosome; nucleus

Molecular Function: protein binding

Biological Process: skin development; protein deubiquitination; viral reproduction; skeletal morphogenesis; single fertilization; multicellular organism growth; homeostasis of number of cells; embryonic organ development; spermatogenesis; double-strand break repair via homologous recombination; positive regulation of epithelial cell proliferation