Rabbit anti-Human WDR45 Polyclonal Antibody | anti-WDR45 antibody

WDR45, NT (WDR45, WDRX1, WDRXI4, WIPI4, WD repeat domain phosphoinositide-interacting protein 4, WD repeat-containing protein 45) (FITC)

Cat. Num. AAA6363283

• Gene Names: WDR45; JM5; NBIA4; NBIA5; WDRX1; WIPI4; WIPI-4
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: WDR45; Polyclonal Antibody; NT (WDR45; WDRX1; WDRXI4; WIPI4; WD repeat domain phosphoinositide-interacting protein 4; WD repeat-containing protein 45) (FITC); WD repeat-containing protein 45; anti-WDR45 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-WDR45 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: WDR45 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-46 amino acids from the N-terminal region of human WDR45.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(WDR45 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the WDR45 antibody detected the WDR45 protein (arrow).)

Related Product Information for anti-WDR45 antibody

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene has a pseudogene at chromosome 4q31.3. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity and full-length nature of some variants have not been determined.

Product Categories/Family for anti-WDR45 antibody

Antibodies; WD Repeat Proteins

References

Proikas-Cezanne, T., et al. Oncogene 23(58):9314-9325(2004). Jeffries, T.R., et al. Mol. Biol. Cell 15(6):2652-2663(2004). Clark, A.G., et al. Science 302(5652):1960-1963(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 47059042
• NCBI GeneID: 11152
• NCBI Accession #: NP_009006.2; NP_001025067.1
• NCBI GenBank Nucleotide #: NM_007075.3
• UniProt Accession #: Q9Y484; Q5MNZ5; Q6IBS7; Q6NT94; Q96H03; A6NGH5; B7WPI2
• Molecular Weight: 39,939 Da
• NCBI Official Full Name: WD repeat domain phosphoinositide-interacting protein 4 isoform 1
• NCBI Official Synonym Full Names: WD repeat domain 45
• NCBI Official Symbol: WDR45
• NCBI Official Synonym Symbols: JM5; NBIA4; NBIA5; WDRX1; WIPI4; WIPI-4
• NCBI Protein Information: WD repeat domain phosphoinositide-interacting protein 4
• UniProt Protein Name: WD repeat domain phosphoinositide-interacting protein 4
• Protein Family: WD repeat domain phosphoinositide-interacting protein
• UniProt Gene Name: WDR45
• UniProt Synonym Gene Names: WDRX1; WDRXI4; WIPI4; WIPI-4

NCBI Description

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene has a pseudogene at chromosome 4q31.3. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity and full-length nature of some variants have not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Plays an important role in the autophagy pathway, which is the major intracellular degradation system by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation.