Rabbit WC1 / ATP7B Polyclonal Antibody | anti-WC1 antibody

Rabbit Polyclonal (IgG) to Human WC1 / ATP7B

Cat. Num. AAA243682

• Gene Names: ATP7B; WD; PWD; WC1; WND
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: WC1 / ATP7B; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human WC1 / ATP7B; Anti-WC1 / ATP7B Antibody (aa161-210) IHC-plus; ATP7B; Copper-transporting ATPase 2; WC1; Wilson disease; PWD; WD; Copper pump 2; WND; Human WC1; anti-WC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ATP7B Antibody detects endogenous levels of total ATP7B protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1465

• Applicable Applications for anti-WC1 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: ELISA (1:1000), IF (1:100 - 1:500), IHC-P (10 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human ATP7B (aa161-210).
• Immunogen: WC1 / ATP7B antibody was raised against synthetic peptide from human ATP7B (aa161-210).
• Antigen Modification: aa161-210
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-ATP7B antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of HeLa cells, using ATP7B Antibody. The picture on the right is treated with the synthesized peptide.)

Related Product Information for anti-WC1 antibody

ATP7b (ATPase, Cu++ Transporting, Beta Polypeptide), also known as Wilson Disease gene (WND) or mouse toxic milk gene, plays a key role in copper transport. Germ-line mutations in this gene cause so-called Wilson disease, which is characterized by the accumulation of toxic levels of copper in the liver, brain, and kidney resulting in extensive tissue damage and eventual death. ATP7b is expressed in a variety of carcinomas and appears to be associated with chemoresistance to cisplatin.

Product Categories/Family for anti-WC1 antibody

Family: Transporter; Subfamily: ATPase - P type; type IB

NCBI and Uniprot Product Information

• NCBI GI #: 55743071
• NCBI GeneID: 540
• NCBI Accession #: NP_000044.2
• NCBI GenBank Nucleotide #: NM_000053.3
• UniProt Accession #: P35670; Q16318; Q16319; Q4U3V3; Q59FJ9; Q5T7X7
• Molecular Weight: 155,125 Da
• NCBI Official Full Name: copper-transporting ATPase 2 isoform a
• NCBI Official Synonym Full Names: ATPase, Cu++ transporting, beta polypeptide
• NCBI Official Symbol: ATP7B
• NCBI Official Synonym Symbols: WD; PWD; WC1; WND
• NCBI Protein Information: copper-transporting ATPase 2; copper pump 2; Wilson disease-associated protein; ATPase, Cu(2+)- transporting, beta polypeptide
• UniProt Protein Name: Copper-transporting ATPase 2
• UniProt Gene Name: ATP7B
• UniProt Synonym Gene Names: PWD; WC1; WND
• UniProt Entry Name: ATP7B_HUMAN

NCBI Description

This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile. Alternate transcriptional splice variants, encoding different isoforms with distinct cellular localizations, have been characterized. Mutations in this gene have been associated with Wilson disease (WD). [provided by RefSeq, Jul 2008]

Uniprot Description

ATP7B: Involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile. Monomer. Interacts with COMMD1/MURR1. Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Hydrolase; Transporter; Vesicle; Membrane protein, integral; EC 3.6.3.54; Transporter, ion channel

Chromosomal Location of Human Ortholog: 13q14.3

Cellular Component: Golgi membrane; membrane; mitochondrion; integral to plasma membrane; basolateral plasma membrane; perinuclear region of cytoplasm; late endosome; cytoplasmic membrane-bound vesicle; trans-Golgi network

Molecular Function: protein binding; copper ion binding; copper-exporting ATPase activity; ATP binding

Biological Process: lactation; cellular copper ion homeostasis; response to copper ion; metabolic process; sequestering of calcium ion; cellular zinc ion homeostasis; copper ion import; copper ion transport; transmembrane transport; copper ion export; intracellular copper ion transport

Disease: Wilson Disease