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Western Blot (WB) (Anti-WBSCR22 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: HCT116 Whole Cell Lysate Lane C: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:34 kDa Observed band size:34 kDa (We are unsure as to the identity of these extra bands.))

Rabbit anti-Human WBSCR22 Polyclonal Antibody | anti-BUD23 antibody

Anti-WBSCR22 Antibody, Rabbit Polyclonal

Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
WBSCR22; Polyclonal Antibody; Anti-WBSCR22 Antibody; Rabbit Polyclonal; WBSCR22 Antibody; Rabbit PAb; Antigen Affinity Purified; Williams Beuren syndrome chromosome region 22; Anti-HASJ4442 Antibody; Anti-HUSSY-3 Antibody; Anti-MERM1 Antibody; Anti-PP3381 Antibody; Anti-WBMT Antibody; anti-BUD23 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human WBSCR22
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-BUD23 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:50-1:200
IP: 1-5uL/mg of lysate
Immunogen
E Coli-derived Human WBSCR22 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human WBSCR22 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-WBSCR22 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: HCT116 Whole Cell Lysate Lane C: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:34 kDa Observed band size:34 kDa (We are unsure as to the identity of these extra bands.))

Western Blot (WB) (Anti-WBSCR22 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: HCT116 Whole Cell Lysate Lane C: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:34 kDa Observed band size:34 kDa (We are unsure as to the identity of these extra bands.))

Immunohistochemistry (IHC)

(Immunochemical staining of human WBSCR22 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC) (Immunochemical staining of human WBSCR22 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human WBSCR22 in human pancreas with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC) (Immunochemical staining of human WBSCR22 in human pancreas with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunoprecipitation (IP)

(WBSCR22 was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate 4 uL anti-WBSCR22 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-WBSCR22 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa Observed band size :34 kDa)

Immunoprecipitation (IP) (WBSCR22 was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate 4 uL anti-WBSCR22 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-WBSCR22 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa Observed band size :34 kDa)
Related Product Information for anti-BUD23 antibody
One of the proteins involved in modifying ribosomes so they are functional is called BUD23. BUD23 in Saccharomyces cerevisiae encodes for a class I methyltransferase, and deletion of the gene results in slow growth and random budding phenotypes. Deletion of BUD23 leads to reduced levels of the small (40S) ribosomal subunit, and a block in processing 20S rRNA to 18S rRNA, a late step in 40S maturation. Bud23 protein, but not its methyltransferase activity, is important for biogenesis and export of the 40S subunit in yeast. Baxter et al. found that ribosomes in human cells with no BUD23 were different than in normal cells and that cells without BUD23 produced different proteins, which did not always perform their roles correctly. Proteins in the mitochondria are one of the main groups affected by the absence of BUD23.
References
White J, et al. (2008) Bud23 methylates g1575 of 18s rrna and is required for efficient nuclear export of pre-40s subunits. Mol Cell Biol 28 (10): 3151-3161.Lin JL, et al. (2012) New phenotypes generated by the g57r mutation of bud23 in saccharomyces cerevisiae. Yeast 29 (12): 537-546.Baxter M, et al. (2020) Cardiac mitochondrial function depends on bud23 mediated ribosome programming. Elife 9: e50705.

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Product Notes

The BUD23 (Catalog #AAA8108156) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-WBSCR22 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's WBSCR22 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC-P: 1:50-1:200 IP: 1-5uL/mg of lysate. Researchers should empirically determine the suitability of the BUD23 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WBSCR22, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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