Rabbit WBSCR22 Polyclonal Antibody | anti-BUD23 antibody

WBSCR22 Antibody

Cat. Num. AAA9629452

• Gene Names: WBSCR22; WBMT; MERM1; PP3381; HUSSY-3; HASJ4442
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: WBSCR22; Polyclonal Antibody; WBSCR22 Antibody; HASJ4442; HUSSY 3; HUSSY3; PP3381; Uncharacterized methyltransferase WBSCR22; WBMT; WBS22_HUMAN; Williams Beuren candidate region putative methyltransferase; Williams Beuren syndrome chromosome region 22 protein; Williams-Beuren syndrome chromosomal region 22 protein; anti-BUD23 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: WBSCR22 Antibody detects endogenous levels of total WBSCR22.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-BUD23 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Similarity: Belongs to the class I-like SAM-binding methyltransferase superfamily. BUD23/WBSCR22 family.
• Post Translational Modifications: May be ubiquitinated and targeted to degradation in response to proinflammatory cytokine signaling.
• Subunit Structure: Heterodimer with TRMT112; this heterodimerization is necessary for the metabolic stability and activity of the catalytic subunit BUD23. Interacts with GRIP1.
• Tissue Specificity: Widely expressed, with high levels in heart, skeletal muscle and kidney. Detected at high levels in bronchial brushings and in normal lung (at protein level). In fetal lung tissue, expressed in the developing bronchial lumen lining cells (at protein level). Tends to be down-regulated in lungs affected by inflammatory diseases or neoplasia (at protein level). Expressed in immune cells, including B and T lymphocytes and macrophages.
• Function: S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N(7) position of a guanine in 18S rRNA. Requires the methyltransferase adapter protein TRM112 for full rRNA methyltransferase activity. Involved in the pre-rRNA processing steps leading to small-subunit rRNA production independently of its RNA-modifying catalytic activity. Important for biogenesis end export of the 40S ribosomal subunit independent on its methyltransferase activity. Locus-specific steroid receptor coactivator. Potentiates transactivation by glucocorticoid (NR3C1), mineralocorticoid (NR3C2), androgen (AR) and progesterone (PGR) receptors. Required for the maintenance of open chromatin at the TSC22D3/GILZ locus to facilitate NR3C1 loading on the response elements. Required for maintenance of dimethylation on histone H3 'Lys-79' (H3K79me2), although direct histone methyltransferase activity is not observed in vitro.
• Note: Localized diffusely throughout the nucleus and the cytoplasm (PubMed:24488492). Localizes to a polarized perinuclear structure, overlapping partially with the Golgi and lysosomes (PubMed:25851604). Localization is not affected by glucocorticoid treatment (PubMed:24488492).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-BUD23 antibody

Nucleus; Nucleoplasm; Cytoplasm; Perinuclear region; Cytoplasm; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 321400149
• NCBI GeneID: 114049
• NCBI Accession #: NP_001189489
• NCBI GenBank Nucleotide #: NM_001202560
• UniProt Accession #: O43709; Q96P12; Q9BQ58; Q9HBP9; A8K501; C9K060
• Molecular Weight: 33,846 Da
• NCBI Official Full Name: probable 18S rRNA (guanine-N(7))-methyltransferase isoform 1
• NCBI Official Synonym Full Names: Williams Beuren syndrome chromosome region 22
• NCBI Official Symbol: WBSCR22
• NCBI Official Synonym Symbols: WBMT; MERM1; PP3381; HUSSY-3; HASJ4442
• NCBI Protein Information: probable 18S rRNA (guanine-N(7))-methyltransferase; probable 18S rRNA (guanine-N(7))-methyltransferase; Williams-Beuren candidate region putative methyltransferase; Williams-Beuren syndrome chromosomal region 22 protein; bud site selection protein 23 homo
• UniProt Protein Name: Probable 18S rRNA (guanine-N(7))-methyltransferase
• Protein Family: 18S rRNA
• UniProt Gene Name: WBSCR22
• UniProt Synonym Gene Names: MERM1
• UniProt Entry Name: WBS22_HUMAN