Rabbit anti-Human VPS39 Polyclonal Antibody | anti-VPS39 antibody

VPS39 (Vam6/Vps39-like Protein, KIAA0770, TRAP1-like Protein, TLP, VAM6, hVam6p) (HRP)

Cat. Num. AAA6398363

• Gene Names: VPS39; TLP; VAM6; hVam6p
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: VPS39; Polyclonal Antibody; VPS39 (Vam6/Vps39-like Protein; KIAA0770; TRAP1-like Protein; TLP; VAM6; hVam6p) (HRP); anti-VPS39 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human VPS39.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 4851

• Applicable Applications for anti-VPS39 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human VPS39, aa1-875 (NP_056104.2).
• Immunogen Sequence: MHDAFEPVPILEKLPLQIDCLAAWEEWLLVGTKQGHLLLYRIRKDVGCNRFEVTLEKSNKNFSKKIQQIHVVSQFKILVSLLENNIYVHDLLTFQQITTVSKAKGASLFTCDLQHTETGEEVLRMCVAVKKKLQLYFWKDREFHELQGDFSVPDVPKSMAWCENSICVGFKRDYYLIRVDGKGSIKELFPTGKQLEPLVAPLADGKVAVGQDDLTVVLNEEGICTQKCALNWTDIPVAMEHQPPYIIAVLPRYVEIRTFEPRLLVQSIELQRPRFITSGGSNIIYVASNHFVWRLIPVPMATQIQQLLQDKQFELALQLAEMKDDSDSEKQQQIHHIKNLYAFNLFCQKRFDESMQVFAKLGTDPTHVMGLYPDLLPTDYRKQLQYPNPLPVLSGAELEKAHLALIDYLTQKRSQLVKKLNDSDHQSSTSPLMEGTPTIKSKKKLLQIIDTTLLKCYLHTNVALVAPLLRLENNHCHIEESEHVLKKAHKYSELIILYEKKGLHEKALQVLVDQSKKANSPLKGHERTVQYLQHLGTENLHLIFSYSVWVLRDFPEDGLKIFTEDLPEVESLPRDRVLGFLIENFKGLAIPYLEHIIHVWEETGSRFHNCLIQLYCEKVQGLMKEYLLSFPAGKTPVPAGEEEGELGEYRQKLLMFLEISSYYDPGRLICDFPFDGLLEERALLLGRMGKHEQALFIYVHILKDTRMAEEYCHKHYDRNKDGNKDVYLSLLRMYLSPPSIHCLGPIKLELLEPKANLQAALQVLELHHSKLDTTKALNLLPANTQINDIRIFLEKVLEENAQKKRFNQVLKNLLHAEFLRVQEERILHQQVKCIITEEKVCMVCKKKIGNSAFARYPNGVVVHYFCSKEVNPADT S
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of VPS39 expression in transfected 293T cell line by VPS39 polyclonal antibody. Lane 1: VPS39 transfected lysate (100.8kD). Lane 2: Non-transfected lysate.)

Product Categories/Family for anti-VPS39 antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 54234023
• NCBI GeneID: 23339
• NCBI Accession #: NM_015289.2
• NCBI GenBank Nucleotide #: NM_015289.2
• UniProt Accession #: Q96JC1
• NCBI Official Full Name: Homo sapiens vacuolar protein sorting 39 homolog (S. cerevisiae) (VPS39), mRNA
• NCBI Official Synonym Full Names: VPS39 subunit of HOPS complex
• NCBI Official Symbol: VPS39
• NCBI Official Synonym Symbols: TLP; VAM6; hVam6p
• NCBI Protein Information: vam6/Vps39-like protein
• UniProt Protein Name: Vam6/Vps39-like protein
• Protein Family: Vam6/Vps39-like protein
• UniProt Gene Name: VPS39
• UniProt Synonym Gene Names: KIAA0770; TLP; VAM6; hVam6p
• UniProt Entry Name: VPS39_HUMAN

NCBI Description

This gene encodes a protein that may promote clustering and fusion of late endosomes and lysosomes. The protein may also act as an adaptor protein that modulates the transforming growth factor-beta response by coupling the transforming growth factor-beta receptor complex to the Smad pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

VPS39: May play a role in clustering and fusion of late endosomes and lysosomes. Regulator of TGF-beta/activin signaling, inhibiting SMAD3- and activating SMAD2-dependent transcription. Acts by interfering with SMAD3/SMAD4 complex formation, this would lead to inhibition of SMAD3-dependent transcription and relieve SMAD3 inhibition of SMAD2-dependent promoters, thus increasing SMAD2-dependent transcription. Does not affect TGF-beta-induced SMAD2 or SMAD3 phosphorylation, nor SMAD2/SMAD4 complex formation. Belongs to the VAM6/VPS39 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, peripheral; G protein regulator, misc.

Chromosomal Location of Human Ortholog: 15q15.1

Cellular Component: lysosomal membrane; late endosome membrane

Biological Process: vesicle-mediated transport; intracellular protein transport; regulation of catalytic activity