Rabbit anti-Human VPS26 Polyclonal Antibody | anti-VPS26 antibody

VPS26 Rabbit pAb

Cat. Num. AAA4754931

• Gene Names: VPS26A; HB58; PEP8A; VPS26; Hbeta58
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: VPS26; Polyclonal Antibody; VPS26 Rabbit pAb; HB58; PEP8A; Hbeta58; anti-VPS26 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Affinity purified
• Form/Format: -20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

• Applicable Applications for anti-VPS26 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Synthetic peptide of human VPS26A
• Conjugation: Unconjugated
• Molecular Function: This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Modification: Unmodified
• Cell Position: Cytoplasm, Endosome, Membrane
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane 1-7: Human placenta tissue, PC3 cells, Human fetal liver tissue, HepG2 cells, Hela cells, A431 cells, 293T cells, Primary antibody: 162484(VPS26A Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 40 seconds)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 162484(VPS26A Antibody) at dilution 1/35. (Original magnification: ×200))

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using 162484(VPS26A Antibody) at dilution 1/35. (Original magnification: ×200))

Product Categories/Family for anti-VPS26 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 17978519
• NCBI GeneID: 9559
• NCBI Accession #: NP_004887
• NCBI GenBank Nucleotide #: NM_004896.4
• UniProt Accession #: O75436; Q8TBH4; Q9H982; A8MZ56; B2RDD3
• Molecular Weight: 40.6 kDa (350aa), confirmed by MALDI-TOF
• NCBI Official Full Name: vacuolar protein sorting-associated protein 26A isoform 1
• NCBI Official Synonym Full Names: VPS26, retromer complex component A
• NCBI Official Symbol: VPS26A
• NCBI Official Synonym Symbols: HB58; PEP8A; VPS26; Hbeta58
• NCBI Protein Information: vacuolar protein sorting-associated protein 26A
• UniProt Protein Name: Vacuolar protein sorting-associated protein 26A
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: VPS26A
• UniProt Synonym Gene Names: VPS26; hVPS26
• UniProt Entry Name: VP26A_HUMAN

NCBI Description

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

VPS26A: Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Belongs to the VPS26 family.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 10q21.1

Cellular Component: intracellular membrane-bound organelle; retromer complex; early endosome; endosome membrane; cytosol; endosome; vesicle

Molecular Function: protein binding; protein transporter activity

Biological Process: intracellular protein transport; retrograde transport, endosome to Golgi