Sheep Vitronectin Polyclonal Antibody | anti-Vtn antibody

Sheep anti-human Vitronectin, Peroxidase Conjugated Affinity-Purified IgG

Cat. Num. AAA512173

• Gene Names: Vtn; Vn; AI256434
• Purity: Affinity purified, peroxidase conj. IgG.; Vial containing ml of affinity-purified IgG conjugated to horseradish peroxidase (HRP) through carbohydrate groups. Total protein is 0.1 mg.
• Synonyms: Vitronectin; Polyclonal Antibody; Sheep anti-human Vitronectin; Peroxidase Conjugated Affinity-Purified IgG; human; anti-Vtn antibody

• Host: Sheep
• Clonality: Polyclonal
• Specificity: Prior to conjugation, this antibody was specific for vitronectin as demonstrated by immunoelectrophoresis and ELISA.
• Purity/Purification: Affinity purified, peroxidase conj. IgG.; Vial containing ml of affinity-purified IgG conjugated to horseradish peroxidase (HRP) through carbohydrate groups. Total protein is 0.1 mg.
• Form/Format: APIgG-HRP conjugate as a clear, slightly red-brown liquid.
• Concentration: APIgG-HRP concentration is mg/ml, determined by absorbance using an extinction coefficient (E1%280) of 14. (varies by lot)

• Application Notes: Suitable as a source of peroxidase-labeled antibodies to vitronectin.
• Immunogen: Human Vitronectin purified from plasma.
• Buffer: A buffered stabilizer solution containing 50% (v/v) glycerol.
• Preparation and Storage: Store between -10 and -20 degree C. Product will become viscous but will not freeze. Avoid storage in frost-free freezers. Keep vial tightly capped. Allow product to warm to room temperature and gently mix before use. Avoid exposure to sodium azide as this is an inhibitor of peroxidase activity.

Related Product Information for anti-Vtn antibody

Vitronectin (Vn), previously known as serum-spreading factor or Sprotein, is a plasma and serum glycoprotein with a normal concentration ranging from 200 - 400 ug/ml. It exists in both a 75 kDa single-chain form and a 65 + 10 kDa two-chain form. Vn can exist in a least two different conformational forms. The majority of Vn found in the circulation is present in the native ("closed") form. In this form, most of the binding sites for other ligands are cryptic. The second form of Vn, the denatured ("open", multimeric) form, is a result of a conformational change in the native protein induced by denaturants such as urea, adsorption onto surfaces, low pH or reduction and alkylation. This conformational change leads to exposure of the heparin binding site, formation of disulfide-bonded multimers and rupture of the disulfide bond that links the 10 kDa light chain to the 65 kDa heavy chain of the two chain form. The liver is the primary site of vitronectin synthesis, however, Vn is also found in platelets, megakaryocytes, monocytes and macrophages. Vn plays an important role in a number of physiological and pathophysiological processes. It promotes the adhesion and spreading of a wide variety of cell types and is a subcomponent of the soluble SC5b-9 complex of complement where it protects bystander cells from cytolysis. Vn also plays an important role in fibrinolysis by stabilizing PAI-1 in its active conformation which otherwise rapidly converts to a latent form.1-3

References

1. Tomasini, B.R., and Mosher, D.F. Vitronectin. Prog. Hemost. Thromb., 10:269-305, 1991.
2. Hess, S., Stockmann, A., Voler, W., and Preissner, K.T. Multimeric vitronectin: structure and function. In: Biology of Vitronectins and their Receptors, Elsevier Science Publishers, Amsterdam, p. 21-29, 1993.
3. Preissner, K.T., and Jenne, D. Vitronectin: a new molecular connection in haemostasis. Thrombo. Haemost., 66(2):189-194, 1991.

NCBI and Uniprot Product Information

• NCBI GI #: 6755987
• NCBI GeneID: 22370
• NCBI Accession #: NP_035837.1
• NCBI GenBank Nucleotide #: NM_011707.1
• UniProt Accession #: P29788; Q5SYG4; Q8VII4; Q91X32; Q9D080
• Molecular Weight: 52752 Da
• NCBI Official Full Name: vitronectin
• NCBI Official Synonym Full Names: vitronectin
• NCBI Official Symbol: Vtn
• NCBI Official Synonym Symbols: Vn; AI256434
• NCBI Protein Information: vitronectin; S-protein; OTTMUSP00000000198; serum-spreading factor
• UniProt Protein Name: Vitronectin
• Protein Family: Vitronectin
• UniProt Gene Name: Vtn
• UniProt Entry Name: VTNC_MOUSE

Uniprot Description

Function: Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.

Subunit structure: Interacts with SERPINE1/PAI1 and insulin

Subcellular location: Secreted › extracellular space.

Tissue specificity: Plasma.

Domain: The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin

Post-translational modification: Sulfated on 2 tyrosine residues

By similarity.N- and O-glycosylated

By similarity. Ref.10It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.

Sequence similarities: Contains 4 hemopexin-like domains.Contains 1 SMB (somatomedin-B) domain.