Rabbit anti-Mouse Vitamin D Binding Protein (DBP) Polyclonal Antibody | anti-DBP antibody

Polyclonal Antibody to Vitamin D Binding Protein (DBP)

Cat. Num. AAA2003735

• Gene Names: Gc; DBP; VDB
• Reactivity: Mouse
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Vitamin D Binding Protein (DBP); Polyclonal Antibody; Polyclonal Antibody to Vitamin D Binding Protein (DBP); anti-DBP antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against DBP. It has been selected for its ability to recognize DBP in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.; MGHHHHHHSGS-QMKHLS LLTTMSNRVC SQYAAYGKEK SRLSHLIKLA QKVPTANLEN VLPLAEDFTE ILSRCCESTS EDCMASELPE HTIKICQNLS KKNSKFEECC QENTPMNIFM CTYFMPAAEP LQLPAIKLPT GKDLCGQSTT QAMDQYTFEL SRRTQVPEVF LSKVLEPTLK TLRECCDTQD SVACFSTQSP LLKRQLTSFI EKG
• Sequence Length: 493

• Applicable Applications for anti-DBP antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant DBP (Gln205~Gly403) expressed in E.coli.
• Cross Reactivity: Mouse
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2059379
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Mouse Testis lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse DBP Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P. Samples: Mouse Tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 51172612
• NCBI GeneID: 14473
• NCBI Accession #: NP_032122.1
• NCBI GenBank Nucleotide #: NM_008096.2
• UniProt Accession #: P21614; Q7TS97; Q91XG1; Q9CY31
• Molecular Weight: 53,600 Da
• NCBI Official Full Name: vitamin D-binding protein
• NCBI Official Synonym Full Names: group specific component
• NCBI Official Symbol: Gc
• NCBI Official Synonym Symbols: DBP; VDB
• NCBI Protein Information: vitamin D-binding protein
• UniProt Protein Name: Vitamin D-binding protein
• Protein Family: Remorin
• UniProt Gene Name: Gc
• UniProt Synonym Gene Names: DBP; VDB

Uniprot Description

GC: Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5 E1|5 44.32 cM

Cellular Component: axon; cytosol; extracellular region; extracellular space; perinuclear region of cytoplasm

Molecular Function: actin binding; vitamin D binding; vitamin transporter activity

Biological Process: transport; vitamin D metabolic process; vitamin transport