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Western Blot (WB) (Western blot analysis of VIP antibody (C-term) in Jurkat cell line lysates (35ug/lane). VIP (arrow) was detected using the purified Pab.)

Rabbit anti-Human VIP Polyclonal Antibody | anti-VIP antibody

VIP, CT (VIP Peptides, Intestinal Peptide PHV-42, Intestinal Peptide PHM-27, Peptide Histidine Methioninamide 27, Vasoactive Intestinal Peptide, VIP, Vasoactive Intestinal Polypeptide) (APC)

Gene Names
VIP; PHM27
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
VIP; Polyclonal Antibody; CT (VIP Peptides; Intestinal Peptide PHV-42; Intestinal Peptide PHM-27; Peptide Histidine Methioninamide 27; Vasoactive Intestinal Peptide; Vasoactive Intestinal Polypeptide) (APC); anti-VIP antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human VIP.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-VIP antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-1:100
ELISA: 1:1,000
WB: 1:50-1:100
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human VIP (KLH).
Conjugate
APC
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of VIP antibody (C-term) in Jurkat cell line lysates (35ug/lane). VIP (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of VIP antibody (C-term) in Jurkat cell line lysates (35ug/lane). VIP (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human prostate carcinoma with VIP Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human prostate carcinoma with VIP Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of jurkat cells using VIP Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of jurkat cells using VIP Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-VIP antibody
References
1. Lu, Y., Neurogastroenterol. Motil. 21(7), 754-E47 (2009). 2. Zhu, L., Int J Gynaecol Obstet 105(3), 223-225 (2009).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
19,082 Da
NCBI Official Full Name
VIP peptides isoform 1 preproprotein
NCBI Official Synonym Full Names
vasoactive intestinal peptide
NCBI Official Symbol
VIP
NCBI Official Synonym Symbols
PHM27
NCBI Protein Information
VIP peptides
UniProt Protein Name
VIP peptides
Protein Family
UniProt Gene Name
VIP
UniProt Synonym Gene Names
VIP
UniProt Entry Name
VIP_HUMAN

NCBI Description

The protein encoded by this gene belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Nov 2014]

Uniprot Description

VIP: VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder. Belongs to the glucagon family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6q25

Cellular Component: cell soma; extracellular region

Molecular Function: neuropeptide hormone activity; protein binding; hormone activity

Biological Process: negative regulation of smooth muscle cell proliferation; mRNA stabilization; negative regulation of potassium ion transport; regulation of signal transduction; positive regulation of vasodilation; body fluid secretion; G-protein coupled receptor protein signaling pathway; regulation of protein localization; learning and/or memory; positive regulation of cell proliferation; positive regulation of protein catabolic process; positive regulation of endothelial cell proliferation; regulation of sensory perception of pain; negative regulation of apoptosis

Research Articles on VIP

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Product Notes

The VIP vip (Catalog #AAA6505641) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The VIP, CT (VIP Peptides, Intestinal Peptide PHV-42, Intestinal Peptide PHM-27, Peptide Histidine Methioninamide 27, Vasoactive Intestinal Peptide, VIP, Vasoactive Intestinal Polypeptide) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's VIP can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-1:100 ELISA: 1:1,000 WB: 1:50-1:100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the VIP vip for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VIP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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