Western Blot (WB)
(Western Blot: Sample. Lane1: Mouse Heart Tissue; Lane2: Human Lung Tissue.)
Rabbit anti-Human Vinculin (VCL) Polyclonal Antibody | anti-VCL antibody
Polyclonal Antibody to Vinculin (VCL)
MGHHHHHHSGSEF-K DIAKASDEVT RLAKEVAKQC TDKRIRTNLL QVCERIPTIS TQLKILSTVK ATMLGRTNIS DEESEQATEM LVHNAQNLMQ SVKETVREAE AASIKIRTDA GFTLRWVRKT PWYQ
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample. Lane1: Mouse Heart Tissue; Lane2: Human Lung Tissue.)
Western Blot (WB)
(Western Blot: Sample. Lane1: Mouse Heart Tissue; Lane2: Human Lung Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant VCL, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant VCL, Human.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded stomach tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded stomach tissue))
NCBI and Uniprot Product Information
NCBI Description
Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
Vinculin: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. Exhibits self-association properties. Interacts with NRAP and SORBS1. Interacts with TLN1. Interacts with SYNM. Interacts with CTNNB1 and this interaction is necessary for its localization to the cell-cell junctions and for its function in regulating cell surface expression of E-cadherin. Metavinculin is muscle-specific. Belongs to the vinculin/alpha-catenin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell adhesion; Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 10q22.2
Cellular Component: adherens junction; cell-cell adherens junction; cell-matrix junction; costamere; cytoskeleton; cytosol; extracellular exosome; extracellular region; fascia adherens; ficolin-1-rich granule lumen; focal adhesion; intercellular junction; podosome; protein complex; sarcolemma; secretory granule lumen
Molecular Function: actin binding; alpha-catenin binding; beta-catenin binding; cadherin binding; protein binding; structural molecule activity; ubiquitin protein ligase binding
Biological Process: apical junction assembly; axon extension; cell adhesion; cell motility; cell-matrix adhesion; epithelial cell-cell adhesion; lamellipodium assembly; morphogenesis of an epithelium; muscle contraction; negative regulation of cell migration; neutrophil degranulation; platelet degranulation; protein localization to cell surface
Disease: Cardiomyopathy, Dilated, 1w
Research Articles on VCL
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Product Notes
The VCL vcl (Catalog #AAA2007218) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Vinculin (VCL) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Vinculin (VCL) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the VCL vcl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-K DIAKASDEVT RLAKEVAKQC TDKRIRTNLL QVCERIPTIS TQLKILSTVK ATMLGRTNIS DEESEQATEM LVHNAQNLMQ SVKETVREAE AASIKIRTDA GFTLRWVRKT PWYQ. It is sometimes possible for the material contained within the vial of "Vinculin (VCL), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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