Rabbit anti-Human, Mouse Vinculin Polyclonal Antibody | anti-VCL antibody

Vinculin, CT (VCL, VINC, CMD1W, CMH15, Metavinculin, MVCL) (MaxLight 405)

Cat. Num. AAA6505821

• Gene Names: VCL; MV; MVCL; CMD1W; CMH15; HEL114
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Vinculin; Polyclonal Antibody; CT (VCL; VINC; CMD1W; CMH15; Metavinculin; MVCL) (MaxLight 405); anti-VCL antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human VINC. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-VCL antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human VINC (KLH).
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-VINC Antibody (C-term) in Ramos cell line lysates (35ug/lane). VINC (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of anti-VINC Antibody (C-term) in mouse lung tissue lysates (35ug/lane). VINC (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-VCL antibody

Antibodies; Membrane Proteins

References

1. Moiseyeva E.P., Weller P.A.J. Biol. Chem. 268:4318-4325(1993) 2. Sun N., Critchley D.R., Paulin D.Biochem. J. 409:657-667(2008). 3. Izard T., Evans G., Borgon R.A.Nature 427:171-175(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 7669550
• NCBI GeneID: 7414
• NCBI Accession #: NP_054706
• NCBI GenBank Nucleotide #: NM_014000.2
• UniProt Accession #: P18206
• Molecular Weight: 124kDa
• NCBI Official Full Name: vinculin isoform meta-VCL
• NCBI Official Synonym Full Names: vinculin
• NCBI Official Symbol: VCL
• NCBI Official Synonym Symbols: MV; MVCL; CMD1W; CMH15; HEL114
• NCBI Protein Information: vinculin
• UniProt Protein Name: Vinculin
• Protein Family: Vinculin
• UniProt Gene Name: VCL
• UniProt Synonym Gene Names: MV
• UniProt Entry Name: VINC_HUMAN

NCBI Description

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Vinculin: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. Exhibits self-association properties. Interacts with NRAP and SORBS1. Interacts with TLN1. Interacts with SYNM. Interacts with CTNNB1 and this interaction is necessary for its localization to the cell-cell junctions and for its function in regulating cell surface expression of E-cadherin. Metavinculin is muscle-specific. Belongs to the vinculin/alpha-catenin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q22.2

Cellular Component: protein complex; costamere; focal adhesion; extracellular region; intercellular junction; fascia adherens; cytosol; cell-matrix junction; actin cytoskeleton; cell-cell adherens junction; adherens junction; cytoskeleton; plasma membrane; vesicle

Molecular Function: protein binding; cadherin binding; beta-catenin binding; structural molecule activity; actin binding; alpha-catenin binding

Biological Process: lamellipodium biogenesis; platelet activation; platelet degranulation; apical junction assembly; muscle contraction; axon extension; cell-matrix adhesion; morphogenesis of an epithelium; blood coagulation; cell adhesion; cell motility; negative regulation of cell migration

Disease: Cardiomyopathy, Dilated, 1w