Sheep Vimentin Polyclonal Antibody | anti-VIM antibody

Sheep anti-human Vimentin, Peroxidase Conjugated Affinity-Purified IgG

Cat. Num. AAA512170

• Gene Names: VIM; FLJ36605
• Purity: Affinity purified, peroxidase conj. IgG.; Vial containing ml of affinity purified IgG conjugated to horseradish peroxidase (HRP) through carbohydrate groups. Total protein is 0.1 mg.
• Synonyms: Vimentin; Polyclonal Antibody; Sheep anti-human Vimentin; Peroxidase Conjugated Affinity-Purified IgG; human; anti-VIM antibody

• Host: Sheep
• Clonality: Polyclonal
• Specificity: Prior to conjugation, this antibody was specific for vimentin as demonstrated by immunoelectrophoresis and ELISA.
• Purity/Purification: Affinity purified, peroxidase conj. IgG.; Vial containing ml of affinity purified IgG conjugated to horseradish peroxidase (HRP) through carbohydrate groups. Total protein is 0.1 mg.
• Form/Format: APIgG-HRP conjugate as a clear, slightly red-brown liquid.
• Concentration: APIgG-HRP concentration is mg/ml, determined by absorbance using an extinction coefficient (E1%280) of 14. (varies by lot)

• Application Notes: Suitable as a source of peroxidase-labeled antibodies to vimentin.
• Immunogen: Purified recombinant human vimentin produced in bacteria. This protein constitutes a soluble 20 kDa fragment from the amino-terminus of vimentin.
• Buffer: A buffered stabilizer solution containing 50% (v/v) glycerol.
• Preparation and Storage: Store at -10 to -20 degree C.

Related Product Information for anti-VIM antibody

Intermediate filaments (IFs), along with microfilaments and microtubules, are the three major filament systems that form the architectural basis of most eukaryotic cells. Vimentin, with a molecular weight of 58 kDa, is a Type III intermediate filament found in cells of mesenchymal origin including endothelial cells, megakaryocytes and platelets as well as in most cultured cell lines. As with other IFs, vimentin provides a physical linkage between the plasma membrane and the nuclear envelope. The carboxy-terminus of vimentin associates with the lamin B of the nuclear envelope whereas the amino-terminus associates with the plasma membrane either directly or indirectly. In general, it is believed that IFs, including vimentin, provide a network onto which various enzyme systems, intracellular structures and organelles are spatially arranged within the cytoplasm. IFs may also play an active role in the re-organization of intracellular components in response to extracellular signals via their disassembly and reassembly. In normal vascular endothelium, vimentin, along with other cytoskeletal proteins, are typically not exposed to extracellular plasma proteins. However, with damage to endothelial cells, exposure of these cytoskeletal components may play a role in thrombogenesis or inflammatory responses. For example, it has been demonstrated that vimentin can bind complement components as wells as immunoglobulins. Vimetin has also been shown to bind vitronectin and vitronectin/PAI-1 complexes in LPS-damaged endothelial cells.1-3

References

1. Steinert, P.M. and Roop, D.R. Molecular and cellular biology of intermediate filaments. Ann. Rev. Biochem. 57:593-625, 1988.
2. Albrect, D.L., Mills, J.W. and Noelle, R.J. Membrane Ig-Cytoskeletal interatctions: receptor cross-linking results in the formation of extensive filamentous arrays of vimentin. J. Immunol. 144:3251-3256, 1990.
3. Podor, T.J. and Loskutoff, D.J. Binding of PAI-1/vitronectin complexes to the intermediate filament cytoskeleton of endotoxin injured endothelial cells. Fibrinolysis (suppl.), 4:263a, 1990.

NCBI and Uniprot Product Information

• NCBI GI #: 62414289
• NCBI GeneID: 7431
• NCBI Accession #: NP_003371.2
• NCBI GenBank Nucleotide #: NM_003380.2
• UniProt Accession #: P08670; Q15867; Q15868; Q15869; Q548L2; Q6LER9; Q8N850; Q96ML2; Q9NTM3; B0YJC2; D3DRU4; Q53HU8
• Molecular Weight: 53521 Da
• NCBI Official Full Name: vimentin
• NCBI Official Synonym Full Names: vimentin
• NCBI Official Symbol: VIM
• NCBI Official Synonym Symbols: FLJ36605
• NCBI Protein Information: vimentin; OTTHUMP00000019224; OTTHUMP00000019225
• UniProt Protein Name: Vimentin
• Protein Family: Vimentin
• UniProt Gene Name: VIM
• UniProt Entry Name: VIME_HUMAN

NCBI Description

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.

Uniprot Description

Function: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.

Subunit structure: Homopolymer. Interacts with HCV core protein. Interacts with LGSN and SYNM. Interacts (via rod region) with PLEC (via CH 1 domain)

By similarity. Interacts with SLC6A4. Ref.22 Ref.35

Tissue specificity: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. Ref.12 Ref.17

Post-translational modification: Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin

By similarity. One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments.

Sequence similarities: Belongs to the intermediate filament family.

Sequence caution: The sequence BAB71275.1 differs from that shown. Reason: Intron retention.