Rabbit VIF Polyclonal Antibody | anti-VIF antibody

Rabbit anti-Human immunodeficiency virus type 1 group M subtype B (isolate N1T-A)(HIV-1) VIF Polyclonal Antibody

• Reactivity: Human immunodeficiency virus type 1 group M subtype B (isolate N1T-A)(HIV-1)
• Applications: ELISA, Western Blot
• Purity: Protein A/G
• Synonyms: VIF; Polyclonal Antibody; Rabbit anti-Human immunodeficiency virus type 1 group M subtype B (isolate N1T-A)(HIV-1) VIF Polyclonal Antibody; VIF Antibody; Virion infectivity factor; Vif; SOR protein; [Cleaved into: p17; p7] vif; anti-VIF antibody

• Host: Rabbit
• Reactivity: Human immunodeficiency virus type 1 group M subtype B (isolate N1T-A)(HIV-1)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Protein A/G
• Form/Format: Liquid; Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH7.4

• Applicable Applications for anti-VIF antibody: ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
• Immunogen: Recombinant Human immunodeficiency virus type 1 group M subtype B (isolate N1T-A) (HIV-1) vif protein
• Conjugation: Non-conjugate
• Modification: Unmodified
• Deliverables: 1. 200ug recombinant immunogen protein/peptide (positive control).; 2. 1mL preimmune serum (negative control); 3. 10mg rabbit polyclonal antibody purified by Protein A/G.
• Quality Guarantee: 1. Antibody purity >90% confirmed by SDS-PAGE.; 2. Antibody titer > 1: 64,000 confirmed by ELISA; 3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
• Complementary Antibody: Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:; * 6*His Monoclonal Antibody #MBS1491670; * DYKDDDDK tag Monoclonal Antibody #MBS1493946; * GAPDH Monoclonal Antibody #MBS1495524; * GFP Monoclonal Antibody #MBS1496050; * Myc tag Monoclonal Antibody #MBS1497694; * GST Monoclonal Antibody #MBS1497871; * Sumo tag Monoclonal Antibody #MBS1498878; * Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510; * HA-Tag Monoclonal Antibody #MBS7135631; * E-Tag Monoclonal Antibody #MBS7135632; * TUBA1A Monoclonal Antibody #MBS7135645; * TUBB Monoclonal Antibody #MBS9018332
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

• NCBI GI #: 401363
• UniProt Accession #: P31820
• Molecular Weight: 22,611 Da
• NCBI Official Full Name: Virion infectivity factor
• UniProt Protein Name: Virion infectivity factor
• Protein Family: Virion infectivity factor
• UniProt Gene Name: vif
• UniProt Synonym Gene Names: Vif

Uniprot Description

Counteracts the innate antiviral activity of host APOBEC3F and APOBEC3G. Forms a complex with host APOBEC3F and APOBEC3G thus preventing the entry of these lethally hypermutating enzymes into progeny virions. Recruits an active E3 ubiquitin ligase complex composed of elongin BC, CUL5, and RBX2 to induce polyubiquitination of APOBEC3G and APOBEC3F. In turn, they are directed to the 26S proteasome for degradation. Vif interaction with APOBEC3G also blocks its cytidine deaminase activity in a proteasome-independent manner, suggesting a dual inhibitory mechanism. May interact directly with APOBEC3G mRNA in order to inhibit its translation. Seems to play a role in viral morphology by affecting the stability of the viral nucleoprotein core. Finally, Vif also contributes to the G2 cell cycle arrest observed in HIV infected cells.