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Immunohistochemistry (IHC) (Immunohistochemistry: Representative staining pattern/morphology rat brain. Tissue pretreated with citrate pH 6, antigen retrieval. MBS620879 diluted to 1:100, IHC-Select Detection with HRP-DAB.)

Rabbit anti-Rat Vesicular GABA Transporter Polyclonal Antibody | anti-VGAT antibody

Vesicular GABA Transporter (solute carrier family 32, member 1, bA122O1.1, GABA and Glycine transporter, hVIAAT, Solute carrier family 32 member 1, Vesicular GABA transporter, Vesicular inhibitory amino acid transporter, VGAT, VIAAT) (PE)

Gene Names
SLC32A1; VGAT; VIAAT
Reactivity
Rat
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Immunoaffinity chromatography.
Synonyms
Vesicular GABA Transporter; Polyclonal Antibody; Vesicular GABA Transporter (solute carrier family 32; member 1; bA122O1.1; GABA and Glycine transporter; hVIAAT; Solute carrier family 32 member 1; Vesicular GABA transporter; Vesicular inhibitory amino acid transporter; VGAT; VIAAT) (PE); anti-VGAT antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes rat VGAT at ~57kD. Species Sequence Homology: human, mouse and monkey.
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-VGAT antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IHC: 1:50-1:500
WB: 1:500-1:1000
Applications are based on unconjugated antibody.
Immunogen
Vesicular GABA Transporter corresponding to the C-terminus, conjugated to KLH. (Acccession #NM_080552.2). Cellular Localization: Cytoplasmic vesicle membrane; Multi-pass membrane protein.
Conjugate
PE
Positive Control
Rat Brain Lysate
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(Immunohistochemistry: Representative staining pattern/morphology rat brain. Tissue pretreated with citrate pH 6, antigen retrieval. MBS620879 diluted to 1:100, IHC-Select Detection with HRP-DAB.)

Immunohistochemistry (IHC) (Immunohistochemistry: Representative staining pattern/morphology rat brain. Tissue pretreated with citrate pH 6, antigen retrieval. MBS620879 diluted to 1:100, IHC-Select Detection with HRP-DAB.)

Western Blot (WB)

(Western Blot Analysis: Rat brain lysate was resolved by electrophoresis, transferred to PVDF and probed with MBS620879 (1:1000). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates VGAT (57kD))

Western Blot (WB) (Western Blot Analysis: Rat brain lysate was resolved by electrophoresis, transferred to PVDF and probed with MBS620879 (1:1000). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates VGAT (57kD))
Product Categories/Family for anti-VGAT antibody
References
1. Tabuchi, Katsuhiko, et al. (2007). Science, 318: 71-6. 2. Chessler, SD et al. (2002). Diabetes, 51:1763-1771 3. McIntire, S.L. et al. (1997). Nature, 389:870-876.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
525
NCBI Official Full Name
Vesicular inhibitory amino acid transporter
NCBI Official Synonym Full Names
solute carrier family 32 (GABA vesicular transporter), member 1
NCBI Official Symbol
SLC32A1
NCBI Official Synonym Symbols
VGAT; VIAAT
NCBI Protein Information
vesicular inhibitory amino acid transporter; hVIAAT; vesicular GABA transporter; GABA and glycine transporter; solute carrier family 32 member 1
UniProt Protein Name
Vesicular inhibitory amino acid transporter
UniProt Gene Name
SLC32A1
UniProt Synonym Gene Names
VGAT; VIAAT; hVIAAT
UniProt Entry Name
VIAAT_HUMAN

NCBI Description

The protein encoded by this gene is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles. The encoded protein is a member of amino acid/polyamine transporter family II. [provided by RefSeq, Jul 2008]

Uniprot Description

SLC32A1: Involved in the uptake of GABA and glycine into the synaptic vesicles. Belongs to the amino acid/polyamine transporter 2 family.

Protein type: Transporter; Membrane protein, integral; Transporter, SLC family; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 20q11.23

Cellular Component: neuron projection; synaptic vesicle membrane; dendrite; plasma membrane; integral to membrane

Molecular Function: glycine transmembrane transporter activity; gamma-aminobutyric acid:hydrogen symporter activity; amino acid transmembrane transporter activity

Biological Process: glycine transport; synaptic transmission; neurotransmitter secretion; ion transport; transmembrane transport; aging; gamma-aminobutyric acid transport

Research Articles on VGAT

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Product Notes

The VGAT slc32a1 (Catalog #AAA6505749) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Vesicular GABA Transporter (solute carrier family 32, member 1, bA122O1.1, GABA and Glycine transporter, hVIAAT, Solute carrier family 32 member 1, Vesicular GABA transporter, Vesicular inhibitory amino acid transporter, VGAT, VIAAT) (PE) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Vesicular GABA Transporter can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). IHC: 1:50-1:500 WB: 1:500-1:1000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the VGAT slc32a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Vesicular GABA Transporter, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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