Rabbit anti-Human VEGFA / VEGF Polyclonal Antibody | anti-VEGFA antibody

Rabbit Polyclonal (IgG) to Human VEGFA / VEGF

Cat. Num. AAA247477

• Gene Names: VEGFA; VPF; VEGF; MVCD1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA, Neutralization Assay
• Purity: Affinity Purified
• Synonyms: VEGFA / VEGF; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human VEGFA / VEGF; Anti-VEGFA / VEGF Antibody IHC-plus; VEGFA; VPF; Vascular permeability factor; VEGF; VEGF-A; MVCD1; Human VEGFA; anti-VEGFA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purified
• Form/Format: PBS, pH 7.2, 30% glycerol, 0.01% Thimerosal.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 395

• Applicable Applications for anti-VEGFA antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA), Neutralization
• Application Notes: ELISA (2 - 3 ug/ml), IHC-P (5 ug/ml), Neut (5 - 10 ug/ml), WB (0.5 - 1 ug/ml)
• Target Species: Human
• Immunogen Description: E Coli expressed recombinant human VEGF.
• Immunogen Type: Recombinant protein
• Immunogen: VEGFA / VEGF antibody was raised against E Coli expressed recombinant human VEGF.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-VEGF antibody IHC of human lung, vascular endothelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.)

Western Blot (WB)

(Western blot of VEGF antibody.)

Related Product Information for anti-VEGFA antibody

VEGFA was originally isolated as a heparin-binding secreted factor from bovine pituitary folliculo-stellate cells. It is a direct acting mitogen specific for vascular endothelial cells and capable of stimulating angiogenesis in vivo. In addition to inducing endothelial cell proliferation it promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Molecular cloning has revealed multiple forms of VEGFA arising from alternative splicing of the RNA transcript.

Product Categories/Family for anti-VEGFA antibody

Family: PDGF

NCBI and Uniprot Product Information

• NCBI GI #: 76781481
• NCBI GeneID: 7422
• NCBI Accession #: NP_003367.4
• NCBI GenBank Nucleotide #: NM_003376.5
• UniProt Accession #: P15692; O60720; O75875; Q074Z4; Q16889; B5BU86; H0Y2S8; H0Y407; H0Y414; H0Y462; H0Y8N2; H3BLW7
• Molecular Weight: 34,406 Da
• NCBI Official Full Name: vascular endothelial growth factor A isoform b
• NCBI Official Synonym Full Names: vascular endothelial growth factor A
• NCBI Official Symbol: VEGFA
• NCBI Official Synonym Symbols: VPF; VEGF; MVCD1
• NCBI Protein Information: vascular endothelial growth factor A; vascular permeability factor
• UniProt Protein Name: Vascular endothelial growth factor A
• UniProt Gene Name: VEGFA
• UniProt Synonym Gene Names: VEGF; VEGF-A; VPF
• UniProt Entry Name: VEGFA_HUMAN

NCBI Description

This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.