Rabbit anti-Human VEGF3 Polyclonal Antibody | anti-VEGF3 antibody

VEGF3, NT (VEGFC, Vascular endothelial growth factor C, Flt4 ligand, Vascular endothelial growth factor-related protein) (PE)

Cat. Num. AAA6362443

• Gene Names: VEGFC; VRP; Flt4-L; LMPH1D
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: VEGF3; Polyclonal Antibody; NT (VEGFC; Vascular endothelial growth factor C; Flt4 ligand; Vascular endothelial growth factor-related protein) (PE); VEGFC; Vascular endothelial growth factor-related protein; anti-VEGF3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human VEGF3.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-VEGF3 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 20-49 in the N-terminal region of human VEGF3.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in MCF-7 cell line lysates (35ug/lane) usingMBS6007314. This demonstrates that MBS6007314 detected the VEGF3 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle using MBS6007314 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6007314 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of MCF-7 cells using MBS6007314 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-VEGF3 antibody

Antibodies; Growth Factors; Cytokines

References

1. Chen, X., et al. Cancer Sci. 101(11):2384-2390(2010). 2. Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010). 3. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 4. Deguchi, K., et al. Anticancer Res. 30(6):2361-2366(2010). 5. Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4885653
• NCBI GeneID: 7424
• NCBI Accession #: NP_005420
• NCBI GenBank Nucleotide #: NM_005429.4
• UniProt Accession #: P49767; B2R9Q8
• Molecular Weight: 14.2kDa (125aa) 18-28KDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: vascular endothelial growth factor C preproprotein
• NCBI Official Synonym Full Names: vascular endothelial growth factor C
• NCBI Official Symbol: VEGFC
• NCBI Official Synonym Symbols: VRP; Flt4-L; LMPH1D
• NCBI Protein Information: vascular endothelial growth factor C
• UniProt Protein Name: Vascular endothelial growth factor C
• UniProt Gene Name: VEGFC
• UniProt Synonym Gene Names: VEGF-C; Flt4-L; VRP
• UniProt Entry Name: VEGFC_HUMAN

NCBI Description

The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. The encoded protein promotes angiogenesis and endothelial cell growth, and can also affect the permeability of blood vessels. The proprotein is further cleaved into a fully processed form that can bind and activate VEGFR-2 and VEGFR-3 receptors. [provided by RefSeq, Apr 2014]

Uniprot Description

VEGFC: Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors. Homodimer; non-covalent and antiparallel. Spleen, lymph node, thymus, appendix, bone marrow, heart, placenta, ovary, skeletal muscle, prostate, testis, colon and small intestine and fetal liver, lung and kidney, but not in peripheral blood lymphocyte. Belongs to the PDGF/VEGF growth factor family.

Protein type: Motility/polarity/chemotaxis; Cell cycle regulation; Secreted, signal peptide; Secreted; Cytokine

Chromosomal Location of Human Ortholog: 4q34.3

Cellular Component: extracellular space; membrane; extracellular region

Molecular Function: protein binding; growth factor activity; vascular endothelial growth factor receptor 3 binding; chemoattractant activity

Biological Process: response to drug; substrate-bound cell migration; platelet activation; positive regulation of neuroblast proliferation; positive regulation of blood vessel endothelial cell migration; signal transduction; positive regulation of protein amino acid autophosphorylation; induction of positive chemotaxis; negative regulation of cell proliferation; organ morphogenesis; positive regulation of angiogenesis; positive regulation of peptidyl-tyrosine phosphorylation; morphogenesis of embryonic epithelium; platelet degranulation; positive regulation of protein secretion; positive chemotaxis; positive regulation of cell division; regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of cell proliferation; angiogenesis; negative regulation of blood pressure; vascular endothelial growth factor receptor signaling pathway; positive regulation of cell-matrix adhesion; blood coagulation; positive regulation of epithelial cell proliferation

Disease: Lymphedema, Hereditary, Id