Western Blot (WB)
(VASH2 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the VASH2 antibody detected the VASH2 protein (arrow).)
Rabbit anti-Human, Mouse VASH2 Polyclonal Antibody | anti-VASH2 antibody
VASH2, CT (VASH2, VASHL, Vasohibin-2, Vasohibin-like protein) (APC)
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
VASH2; Polyclonal Antibody; CT (VASH2; VASHL; Vasohibin-2; Vasohibin-like protein) (APC); Vasohibin-like protein; anti-VASH2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-VASH2 antibody
Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
VASH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 215-245 amino acids from the C-terminal region of human VASH2.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(VASH2 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the VASH2 antibody detected the VASH2 protein (arrow).)
Western Blot (WB)
(VASH2 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the VASH2 antibody detected the VASH2 protein (arrow).)
Western Blot (WB)
(VASH2 Antibody (C-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the VASH2 antibody detected the VASH2 protein (arrow).)
Western Blot (WB)
(VASH2 Antibody (C-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the VASH2 antibody detected the VASH2 protein (arrow).)
Flow Cytometry (FC/FACS)
(VASH2 Antibody (C-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(VASH2 Antibody (C-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-VASH2 antibody
VASH2 is a angiogenesis inhibitor. It inhibits network formation by endothelial cells.
Product Categories/Family for anti-VASH2 antibody
References
Kimura, H., et al. Blood 113(19):4810-4818(2009). Shibuya, T., et al. Arterioscler. Thromb. Vasc. Biol. 26(5):1051-1057(2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,253 Da
NCBI Official Full Name
vasohibin-2 isoform 2
NCBI Official Synonym Full Names
vasohibin 2
NCBI Official Symbol
VASH2
NCBI Protein Information
vasohibin-2; vasohibin-like protein
UniProt Protein Name
Vasohibin-2
Protein Family
UniProt Gene Name
VASH2
UniProt Synonym Gene Names
VASHL
UniProt Entry Name
VASH2_HUMAN
Uniprot Description
VASH2: Angiogenesis inhibitor. Inhibits network formation by endothelial cells. Belongs to the vasohibin family. 5 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 1q32.3
Cellular Component: cytoplasm
Biological Process: positive regulation of angiogenesis; positive regulation of endothelial cell proliferation
Similar Products
Product Notes
The VASH2 vash2 (Catalog #AAA6362291) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The VASH2, CT (VASH2, VASHL, Vasohibin-2, Vasohibin-like protein) (APC) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's VASH2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the VASH2 vash2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VASH2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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